Recent questions tagged biochemistry

Under standard temperature $(T)$ and pressure $(P)$ conditions, $128 \: g$ of an ideal has molecule $A$ occupies a volume of $1 \: L$. The gas molecule $A$ obeys the rela...

A protein without its prosthetic group is known asapoproteinhemoproteinholoproteinlipoprotein

The enzyme which adds phosphate group to the free $5’$ terminus of a $\text{DNA}$ sequence isadenosine kinasealkaline phosphatasepolynucleotide kinaseterminal deoxynucleo...

The enzyme $\alpha$-amylase used in starch hydrolysis has an affinity constant $(K_m)$ value of $0.005 \: M$. To achieve one-fourth of the maximum rate of hydrolysis, the...

Which of the following antimicrobial agent(s) is/are growth factor analog(s)?$5$-FluorouracilIsoniazidSulfanilamideTetracycline

Which of the following chemical messenger(s) is/are derivative(s) of tryptophan?$\gamma$-amino butyric acidIndole acetic acidMelatoninSerotonin

Which of the following nucleus/nuclei is/are $\text{NMR}$ active?$^1H$$^{13}C$$^{16}O$$^{32}S$

A bacterium produces acetic acid from ethanol as per the following reaction $$2CH_3CH_2OH+2O_2 \rightarrow 2CH_3COOH+2H_2O$$ The thermodynamic maximum yield of acetic aci...

The degree of reduction for acetic acid $\left ( C_{2} H_{4}O_{2}\right )$ is ____________.

The mass of $1$ $kmol$ of oxygen molecules is ___________$g$ (rounded off to the nearest integer).

Protein concentration of a crude enzyme preparation was $10\:mg\:mL^{-1}$. $10\:\mu L$ of this sample gave an activity of $5\:\mu mol\:min^{-1}$ under standard assay cond...

In general, which one of the following statements is $NOT \;CORRECT$?Hydrogen bonds result from electrostatic interactionsHydrogen bonds contribute to the folding energy ...

$Group\; I$ lists spectroscopic methods and $Group\; II$ lists biomolecular applications of these methods. Match the methods in $Group\; I$ with the applications in $Grou...

The hexapeptide $P$ has an isoelectric point $(pI)$ of $6.9$. Hexapeptide $Q$ is a variant of $P$ that contains valine instead of glutamate at position $3$. The two pepti...

Which of the following statements are CORRECT about the function of fetal bovine serum in animal cell culture?lt stimulates cell growthIt enhances cell attachmentlt provi...

Which one of the following amino acid residues will destabilize an $\alpha$-helix when inserted in the middle of the helix?ProVallIeLeu

A UV-visible spectrophotometer has a minimum detectable absorbance of $0.02$. The minimum concentration of a protein sample that can be measured reliably in this instrume...

The difference in concentrations of an uncharged solute between two compartments is $1.6$-fold. The energy required for active transport of the solute across the membrane...

Yeast biomass $\left ( C_{6}H_{10}O_{3}N \right )$ grown on glucose is described by the stoichiometric equation given below:$$C_{6}H_{12}O_{6}+0.48\:NH_{3}+3\:O_{2} \to 0...

The number of molecules of a nucleotide of molecular weight $300\:g$/mol present in $10$ picomoles is ____________ $\times10^{12}$ (round off to $2$ decimal places).

A block of ice at $0 \:^{\circ}C$ is supplied heat at a constant rate to convert ice to superheated steam. Which one of the following trajectories correctly represents th...

$W,X$ and $Y$ are the intermediates in a biochemical pathway as shown below:$$S \cdots \cdots \rightarrow W\rightarrow X\rightarrow Y\rightarrow Z$$Mutants auxotrophic fo...

Which of the following strategies are used by cells for metabolic regulation?Phosphorylation-dephosphorylationAllosteryFeedback inhibition$P$ and $Q$ only$P$ and $R$ only...

The sequence of a $1$ $Mb$ long $DNA$ is random. This $DNA$ has all four bases occurring in equal proportion. The number of nucleotides, on average, between two successiv...

Growth of an organism on glucose in a chemostat is characterized by Monod model with specific growth rate = $0.45 \:h^{-1}$ and $K_{s}= 0.5 \:g/L$. Biomass from the subst...

Protein $A$ and protein $B$ form a covalent complex. Gel filtration chromatography of this complex showed a peak corresponding to $200 \:kDa$. $SDS-PAGE$ analysis of this...

The concentrations of $ATP$, $ADP$ and inorganic phosphate in a cell are $2.59, \:0.73$ and $2.72 \:mM$, respectively. Under these conditions, free energy change for the ...

The mitochondrial electron transfer chain oxidizes $NADH$ with oxygen being the terminal electron acceptor. The redox potentials for the two half-reactions are given belo...

Protein $P$ becomes functional upon phosphorylation of a serine residue. Replacing this serine with _____________ will result in a phosphomimic mutant of $P$.alanineaspar...

Ras protein is atrimeric GTPase involved in relaying signal from cell surface to nucleusmonomeric GTPase involved in relaying signal from cell surface to nucleustrimeric ...

Determine the correctness or otherwise of the following Assertion [$a$] and the Reason [$r$] regarding mammalian cells.Assertion [$a$]: Cells use $Ca^{2+}$, and not $Na^{...

Vincristine and vinblastine, two commercially important secondary metabolites from $Catharanthus\; roseus$, are examples ofalkaloidsflavonoidsterpenoidssteroids

Two monomeric His-tagged proteins of identical molecular weight are present in a solution. pIs of these two proteins are $5.6$ and $6.8$. Which one of the following techn...

Which of the following types of molecules act as biological catalysts?Protein$RNA$Phospholipid$P$ and $Q$ only$P$ and $R$ only $Q$ and $R$ only$P, Q$ and $R$

Which of the following are energy transducing membranes?Plasma membrane of bacteriaInner membrane of chloroplastsInner membrane of mitochondria$P$ and $Q$ only$P$ and $R$...

The elemental composition of dry biomass of a yeast species is $CH_{1.6}O_{0.4}N_{0.2}S_{0.0024}P_{0.017}$. The contribution of carbon to the dry biomass is _________ $\%...

Which one of the following is used as a $pH$ indicator in animal cell culture medium ?Acridine orange Phenol redBromophenol blueCoomassie blue

Tetracycline inhibits theinteraction between $tRNA$ and $mRNA$translocation of $mRNA$ through ribosomepeptidyl transferase activitybinding of amino-acyl $tRNA$ to ribosom...

Which one of the following statements is $CORRECT$ for enzyme catalyzed reactions? ( $\Delta G$ is Gibbs free energy change, $K_{eq}$ is equilibrium constant)Enzymes affe...

Which one of the following need NOT be conserved in a biochemical reaction?Total massTotal molesNumber of atoms of each elementTotal energy