Recent questions tagged biochemistry

Match the reagents in $\text{Group I}$ with their preferred cleavage sites in $\text{Group II}$.$\begin{array}{|l|l|l|l|} \hline & \text{Group I} && \text{Group II} \\ \...

$\textit{Saccharomyces cerevisiae}$ produces ethanol by fermentation. The theoretical yield of ethanol from $2.5$ g of glucose is _________ g.

Match the antibiotics in $\text{Group I}$ with their modes of action in $\text{Group II}$.$\begin{array}{|l|l|l|l|} \hline & \text{Group I} && \text{Group II} \\ \hline ...

The $K_i$ of a novel competitive inhibitor designed against an enzyme is $2.5 \: \mu M$. The enzyme was assayed in the absence or presence of the inhibitor $(5 \: \mu M)$...

An $\textit{in vitro}$ translation system can synthesize peptides m all three reading frames of the RNA template. When $\text{5’ - UCUCUCUC - (UC)_n -UCUCUCUC - 3’}$ was...

The standard free energy change $(\Delta G' ^\circ)$ for ATP hydrolysis is $-30 kJ \cdot \text{mole}^{-1}$. The $\textit{in vivo}$ concentrations of ATP, ADP and $P_i$ in...

Cytoplasmic extract from the wild type strain of a bacterium has the ability to convert a colorless substrate $(S)$ to a colored product $(P)$ via three colorless interme...

A linear double stranded DNA of length $8$ kbp has three restriction sites. Each of these can either be a $\textit{Bam}\text{HI}$ or a $\textit{Hae}III$ site. The DNA was...

If the dissociation constant for solute-adsorbent binding is $K_D$, the retention time of the solute in a chromatography columnincreases with increasing $K_D$decreases wi...

The 4-amino or 4-keto group of pyrimidine bases is located in themajor groove of the double stranded DNAminor groove of the double stranded DNAminor groove of the B form ...

The product(s) resulting from the hydrolysis of maltose is/area mixture of $\alpha$-D-Glucose and $\beta$-D-Glucosea mixture of D-Glucose and L-Glucose$\alpha$-D-Glucose ...

Amino acid residue which is most likely to be found in the interior of water-soluble globular proteins isThreonineAspartic acidValineHistidine

The reactions leading to the formation of amino acids from the TCA cycle intermediates arecarboxylationisomerizationtransaminationdecarboxylation

Cholera toxin increases $cAMP$ levels bymodifying $G_i$ proteinmodifying $G_s$ proteinbinding to adenylate cyclaseactivating $cAMP$ phosphodiesterase

Triose phosphate isomerase converts dihydroxy acetone phosphate $(DHAP)$ to glyceraldehyde-3-phosphate $(G-3-P)$ in a reversible reaction. At $\text{298 K and pH 7.0,}$ t...

Match the following photoreceptors with their prosthetic groups and spectral specificity:$\begin{array}{|l|l|l|} \hline \textbf{Photoreceptor} & \textbf{Moiety that absor...

Match the following plant sources with their secondary metabolites and medical uses:$\begin{array}{|l|l|l|} \hline \textbf{Source plant} & \textbf{Secondary metabolites} ...

The pungency of mustard seeds is primarily due to secondary metabolites such as isothiocyanate and nitrile. The pungency is usually felt only when the seeds are crushed. ...

The percentage $\text{SIMILARITIES and IDENTITIES,}$ respectively, between the two peptide sequences given below will be ____________ $\%$ and ____________ $\%$Peptide I ...

The active site in the alpha/beta barrel structures is usually locatedinside the barrelat the amino side of the strandsat the carboxy side of the strandsat any arbitrary ...

A solution was prepared by dissolving $\text{100 mg}$ of protein $X$ in $\text{100 ml}$ of water. Molecular weight of protein $X$ is $\text{15,000 Da};$ Avogadro’s number...

The activity of an enzyme was measured by varying the concentration of the substrate $(S)$ in the presence of three different concentrations of inhibitor $\text{(I) 0, 2 ...

Match the herbicides in $\text{Group I}$ with the target enzymes in $\text{Group II}$ $\begin{array}{|l|l|l|l|} \hline & \text{Group I} && \text{Group II} \\ \hline P. & ...

Match the entries in $\text{Group I}$ with the enzymes in $\text{Group II}$.$\begin{array}{|l|l|l|l|} \hline & \text{Group I} && \text{Group II} \\ \hline P. & \text{NAD}...

Match the antibiotics in $\text{Group I}$ wiith the targets in $\text{Group II}$.$\begin{array}{|l|l|l|l|} \hline & \text{Group I} & & \text{Group II} \\ \hline P. & \t...

The catalytic efficiency for an enzyme is defined as$K_{cat}$$\frac{V_{max}}{K_{cat}}$$\frac{k_{cat}}{K_m}$$\frac{K_{cat}}{V_{max}}$

Which one of the following is an $ABC$ transporter?multidrug resistance proteinacetylcholine receptorbacteriorhodopsin$ATP$ synthase

Which one of the following aminoacids in proteins does $NOT$ undergo phosphorylation?SerThrProTyr

Which one of the following modifications is common toboth protein and DNA?SUMOylationNitrosylationMethylationUbiquitination

$\beta$-Galactosidase bound to DEAE-cellulose is used to hydrolyze lactose to glucose and galactose in a plug flow bioreactor with a packed bed of volume $100$ liters and...

Answer the question based on the information given below:The purification data for an enzyme is given below:$\begin{array}{|l|l|l|l|l|l|} \hline & \text{Step} & \text{Vol...

Answer the question based on the information given below:The purification data for an enzyme is given below:$\begin{array}{|l|l|l|l|l|l|} \hline & \text{Step} & \text{Vol...

$\text{Statement for Linked Answer Questions 52 and 53:}$An E $coli$ cell of volume $10^{-12}$ $cm^3$ contains 60 molecules of lac-repressor. The repressor has a binding ...

$\text{Statement for Linked Answer Questions 52 and 53:}$An E $coli$ cell of volume $10^{-12}$ $cm^3$ contains 60 molecules of lac-repressor. The repressor has a binding ...

In a muscle, the extracellular and intracellular concentrations of ${Na^+}$ are $150 \text{ mM}$ and $12 \text{ mM}$, and those of ${K^+}$ are $2.7 \text{ mM}$ and $140 \...

Match the vitamins in $\text{Group I}$ with the processes/reactions in $\text{Group II}$$\begin{array}{|l|l|l|l|} \hline & \text{Group I} && \text{Group II} \\ \hline P. ...

Match the high energy compounds in $\text{Group I}$ with the biosynhetic pathways for the molecules in $\text{Group II}$.$\begin{array}{|l|l|l|l|} \hline & \text{Group I...

An enzymatic reaction is described by the following rate expression.$$v = \frac{v_mS}{K_m+S+S^2/K_s},$$Which one of the following curves represents this expression?

$\text{Common Data Questions}$$\text{Common Data for Questions 48 and 49}$:In a muscle, the extracellular and intracellular concentrations of ${Na^+}$ are $\text{150 mM}$...