# GATE2014-33

Triose phosphate isomerase converts dihydroxy acetone phosphate $(DHAP)$ to glyceraldehyde-3-phosphate $(G-3-P)$ in a reversible reaction. At $\text{298 K and pH 7.0,}$ the equilibrium mixture contains $\text{40 mM DHAP and 4 mM G-3-P.}$ Assume that the reaction started with $\text{44 mM DHAP and no G-3-P}$. The standard free-energy change in $\text{kJ/mol}$ for the formation of $\text{G-3-P [R = 8.315 J/mol.K]}$ is __________.
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## Related questions

1
A bacterium belonging to cocci group has a diameter of $2\:\mu m.$ The numerical value of the ratio of its surface area to volume (in $\mu m^{-1}$) is _______.
2
A T- ask is seeded with $10^5$ anchorage-dependent cells. The available area of the T- ask is $25\: cm^2$ and the volume of the medium is $25\: ml$. Assume that the cells are rectangles of size $5\: \mu m \times 2\: \mu m$. If the cells grow to monolayer confluence after $50\: h$, the growth rate in number of $cells/(cm^2 .h)$ is _________$\times10^5$
Consider a continuous culture provided with a sterile feed containing $10$ ... $\mu _m$ (in $h^{-1}$), will be _________.
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