Recent questions tagged biochemistry

Which one of the following is NOT a therapeutic agent based on nucleic acid for the treatment of genetic disorders?Antisense oligonucleotideRibozymeAptamerAvidin

ATP biosynthesis takes place utilizing the $H^+$ gradient in mitochondria and chloroplasts. Identify the correct sites of $H^+$ gradient formation.Across the outer membra...

Disaccharide molecules that contain $\beta( 1 –\blacktriangleright 4)$ glycosidic linkage aresucrose and maltosesucrose and isomaltosemaltose and isomaltoselactose and ce...

Which one of the following statements is NOT true?In competitive inhibition, substrate and inhibitor compete for the same active site of an enzymeAddition of a large amou...

Match the secondary metabolites (Column-I) with the corresponding plant species (Column-II).$\begin{array}{|l|l|l|l|} \hline & \textbf{Column-I} & &\textbf{Column-II} \\ ...

The equilibrium potential of a biological membrane for $Na^+$ is $55$ mV at $37^\circ$ C. Concentration of $Na^+$ inside the cell is $20$ mM. Assuming the membrane is per...

Which one of the following statements regarding G proteins is INCORRECT?GDP is bound to G protein in the resting stageGTP bound $\alpha$ subunit cannot reassemble with $\...

In an assay of the type II dehydroquinase of molecular mass $18$ kDa, it is found that the $V_{\text{max}}$ of the enzyme is $0.0134 \: \mu \text{ mol} \cdot \text{min}^{...

The molar extinction coeffcients of Trp and Tyr at $280$ nm are $5690$ and $1280 \: M^{-1} \cdot cm^{-1}$, respectively. The polypeptide chain of yeast alcohol dehydrogen...

The activity of lactate dehydrogenase can be measured by monitoring the following reaction: $$\text{Pyruvate} + \text{NADH} \rightarrow \text{Lactate} + \text{NAD}^+$$ Th...

Analysis of a hexapeptide using enzymatic cleavage reveals the following result:Amino acid composition of the peptide is: $2R, A, V, S, Y$Trypsin digestion yields two fra...

The empirical formula for biomass of an unknown organism is $CH_{1.8}O_{0.5}N_{0.2}$. To grow this organism, ethanol $(C_2H_5OH)$ and ammonia are used as carbon and nitro...

A protein is to be purified using ion-exchange column chromatography. The relationship between HETP (Height Equivalent to Theoretical Plate) and the linear liquid velocit...

An enzyme catalyzes a reaction bydecreasing the energy of the substrate.decreasing the activation energy of the reaction.decreasing product stability.increasing the activ...

Natural proteins are composed primarily of $20$ $\alpha$-amino acids. Which one of the following statements is true for any of these amino acids in a solution of pH $1.5$...

Which one of the following techniques can be used to determine the structure of a $15$ kDa globular protein at atomic resolution?Raman spectroscopyIR spectroscopyUV spect...

The plant hormone indole-$3$-acetic acid is derived fromhistidinetyrosinetryptophanproline

Enzyme-linked immunosorbent assay (ELISA) is used forquantifying antibody levels in blooddetermining the molecular weight of an antigenpurifying proteins from biological ...

If a protein contains four cysteine residues, the number of different ways they can simultaneously form two intra-molecular disulphide bonds is __________.

An enzymatic reaction exhibits Michaelis-Menten kinetics. For this reaction, on doubling the concentration of enzyme while maintaining $[S]>>[E_0]$both $K_m$ and $V_{\tex...

Which one of the following graphs represents the kinetics of protein precipitation by addition of ammonium sulphate? On the $Y$-axis, [Protein] represents the concentrati...

Match the proteins in $\textbf{Group I}$ with cellular processes in $\textbf{Group II}$$\begin{array}{|l|l|l|l|} \hline & \textbf{Group I} & & \text{Group II} \\ \hline P...

The circular dichroism spectra of three proteins $P$, $Q$, and $R$ are given below:Choose the correct combination.$P: \alpha- \text{helix}, Q: \beta- \text{sheet}, \text{...

Which one of the following amino acids has three ionizable groups?GlycineLeucineValineLysine

The concentration (in micromolar) of NADH in a solution with $A_{340}=0.50$ is _________Given data: Path length $=1$ cm; Molar extinction coefficient of NADH $\varepsilon...

The specific activity of an enzyme in a crude extract of $\textit{E.coli}$ is $9.5$ units/mg of protein. The specific activity increased to $68$ units/mg of protein upon ...

Match the plant hormones in $\textbf{Column I}$ with functions in $\textbf{Column II}$$\begin{array}{|l|l|l|l|} \hline & \textbf{Column I} && \text{Column II} \\ \hline ...

If the chemical composition of proteins in an organism is $CH_{1.5}O_{0.3}N_{0.3}S_{0.004}$, the mass percentage of carbon in the protein is __________Given data: Atomic ...

The interaction energy $E$ between two spherical particles is plotted as a function of the distance $(r)$ between them. When $r<a$, where $a$ is a constant, the net force...

A DNA strand of length $25$ nm wraps diametrically around the circumference of a spherical histone-octamer once. The radius (nm) of the histone-octamer is _______Given da...

During anaerobic growth, an organism converts glucose $(P)$ into biomass $(Q)$, ethanol $(R)$, acetaldehyde $(S)$, and glycerol $(T)$. Every mole of carbon present in glu...

Levinthal’s paradox is related toprotein secretionprotein degradationprotein foldingprotein trafficking

ABO blood group antigens in humans are differentiated from each other on the basis ofsialic acidlipidsspectringlycoproteins

A single subunit enzyme converts $420$ $\mu$moles of substrate to product in one minute. The activity of the enzyme is ________ $\times 10^{-6}$ Kalal.

Which one of the following amino acids has the highest probability to be found on the surface of a typical globular protein in aqueous environment?AlaValArgIle

How many $3$-tuples are possible for the following amino acid sequence?MADCMWDISEASE$4$$5$$11$$12$

How many different protein sequences of $100$ residues can be generated using $20$ standard amino acids?$100^{20}$$100 \times 20$$20^{100}$$100! \times 20!$

Which one of the following graphs represents uncompetitive inhibition?

Match the compounds in $\text{Group I}$ with the correct entries in $\text{Group II}$.$\begin{array}{|l|l|l|l|}\hline & \textbf{Group I} & & \textbf{Group II} \\ \hline ...

Match the drugs in $\text{Group I}$ with their mechanism of action in $\text{Group II}$$\begin{array}{|l|l|l|l|} \hline & \textbf{Group I} && \textbf{Group II} \\ \hline ...