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The schematic of a plasmid with a gap in one of the strands is shown below:

Which of the following enzyme(s) is/are required to fill the gap and generate a covalently closed circular plasmid?

  1. $DNA$ ligase
  2. AIkaline phosphatase
  3. $DNA$ polymerase
  4. Polynucleotide kinase
  1. $P$ only
  2. $P, R$ and $S$ only
  3. $P$ and $R$ only
  4. $P, Q$ and $R$ only
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