# GATE2020: 37

The schematic of a plasmid with a gap in one of the strands is shown below:

Which of the following enzyme(s) is/are required to fill the gap and generate a covalently closed circular plasmid?

1. $DNA$ ligase
2. AIkaline phosphatase
3. $DNA$ polymerase
4. Polynucleotide kinase
1. $P$ only
2. $P, R$ and $S$ only
3. $P$ and $R$ only
4. $P, Q$ and $R$ only
in Others
edited

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2
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