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The suitable substitution matrix to align closely related sequences is

  1. $\text{PAM 250 or BLOSSUM 80}$
  2. $\text{PAM 40 or BLOSSUM 80}$
  3. $\text{PAM 120 or BLOSSUM 40}$
  4. $\text{PAM 250 or BLOSSUM 40}$
in Recombinant DNA technology and Other Tools in Biotechnology edited by
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