Most viewed questions in Others / GO BioTechnology

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41

GATE2012-54
$\beta$-Galactosidase bound to DEAE-cellulose is used to hydrolyze lactose to glucose and galactose in a plug flow bioreactor with a packed bed of volume $100$ liters and a voidage ($\epsilon$) of $0.55$. The $K'_m$ and $V'_m$; for the immobilized enzyme are $0.72 \: gl^{-1}$ and ... time required for the steady state reactor operation will be $0.1 \: h$ $0.4 \: h$ $1.0 \: h$ $1.1 \: h$

$\beta$-Galactosidase bound to DEAE-cellulose is used to hydrolyze lactose to glucose and galactose in a plug flow bioreactor with a packed bed of volume $100$ liters and...

Milicevic3306

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Milicevic3306 asked Mar 25, 2018

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42

GATE BT 2023 | Question: 36
The following reaction represents biomass synthesis from hexadecane ... represents the biomass. The value of respiratory quotient (rounded off to two decimal places) is ___________.

The following reaction represents biomass synthesis from hexadecane$\mathrm{C}_{16} \mathrm{H}_{34}+12.5 \mathrm{O}_2+2.13 \mathrm{NH}_3 \rightarrow 10.6 \mathrm{CH}_{1.6...

admin

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admin asked May 21, 2023

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43

GATE BT 2023 | GA Question: 4
In $2022$, June Huh was awarded the Fields medal, which is the highest prize in Mathematics. When he was younger, he was also a poet. He did not win any medals in the International Mathematics Olympiads. He dropped out of college. Based ... of college has won the Fields medal. All Fields medalists are part-time poets. Some Fields medalists have dropped out of college.

In $2022$, June Huh was awarded the Fields medal, which is the highest prize in Mathematics.When he was younger, he was also a poet. He did not win any medals in the Inte...

admin

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admin asked May 21, 2023

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44

GATE2016-13
Which one of the following techniques relies on the spin angular momentum of a photon? CD spectroscopy Fluorescence spectroscopy IR spectroscopy Raman spectroscopy

Which one of the following techniques relies on the spin angular momentum of a photon?CD spectroscopyFluorescence spectroscopyIR spectroscopyRaman spectroscopy

Milicevic3306

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Milicevic3306 asked Mar 26, 2018

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45

GATE BT 2022 | Question: 18
A microorganism is grown in a batch culture using glucose as a carbon source. The apparent growth yield is $0.5 \frac{\text{g biomass}}{\text{g substrate}}.$ ... that there is no endogenous metabolism, the maximum biomass concentration that can be achieved is _________ $ \text{g L}^{-1}.$

A microorganism is grown in a batch culture using glucose as a carbon source. The apparent growth yield is $0.5 \frac{\text{g biomass}}{\text{g substrate}}.$ The initial ...

Lakshman Bhaiya

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Lakshman Bhaiya asked Mar 20, 2022

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46

GATE BT 2023 | Question: 52
The values of the consistency index ' $K$ ' and the flow behavior index ' $n$ ' of a dilatant fluid are 0.415 (in CGS units) and $1.23$, respectively. The value of the apparent viscosity (in $g . \mathrm{cm}^{-1} . \mathrm{s}^{-1}$ ) of this fluid at a shear rate of $60 \mathrm{~s}^{-1}$ (rounded off to the nearest integer) is _____________.

The values of the consistency index ' $K$ ' and the flow behavior index ' $n$ ' of a dilatant fluid are 0.415 (in CGS units) and $1.23$, respectively. The value of the ap...

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admin asked May 21, 2023

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47

GATE2019: 17
Which one of the following is catabolized during endogenous metabolism in a batch bacterial cultivation? internal reserves extracellular substrates extracellular products toxic substrates

Which one of the following is catabolized during endogenous metabolism in a batch bacterial cultivation?internal reservesextracellular substratesextracellular productstox...

soujanyareddy13

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soujanyareddy13 asked Nov 3, 2020

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48

GATE2020: 52
Protein $A$ and protein $B$ form a covalent complex. Gel filtration chromatography of this complex showed a peak corresponding to $200 \:kDa$. $SDS-PAGE$ analysis of this complex, with and without beta-mercaptoethanol, showed a single band corresponding to molecular ... the molecular weight of protein $A$ is $25 \:kDa$, the molecular weight of protein $B$ is ________________ $kDa$.

Protein $A$ and protein $B$ form a covalent complex. Gel filtration chromatography of this complex showed a peak corresponding to $200 \:kDa$. $SDS-PAGE$ analysis of this...

soujanyareddy13

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soujanyareddy13 asked Nov 3, 2020

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49

GATE2020: 22
To facilitate mass transfer from a gas to a liquid phase, a gas bubble of radius $r$ is introduced into the liquid. The gas bubble then breaks into $8$ bubbles of equal radius. Upon this change, the ratio of the interfacial surface area to the gas phase volume for the system changes from $3/r$ to $3n/r$. The value of $n$ is _____________________.

To facilitate mass transfer from a gas to a liquid phase, a gas bubble of radius $r$ is introduced into the liquid. The gas bubble then breaks into $8$ bubbles of equal r...

soujanyareddy13

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soujanyareddy13 asked Nov 3, 2020

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50

GATE2017-54
During anaerobic growth, an organism converts glucose $(P)$ into biomass $(Q)$, ethanol $(R)$, acetaldehyde $(S)$, and glycerol $(T)$ ... , the ethanol produced (in grams) is ________ Given data: Atomic weights (Da) of $C=12, H=1, O=16$, and $N=14$

During anaerobic growth, an organism converts glucose $(P)$ into biomass $(Q)$, ethanol $(R)$, acetaldehyde $(S)$, and glycerol $(T)$. Every mole of carbon present in glu...

Milicevic3306

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Milicevic3306 asked Mar 26, 2018

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51

GATE2016-36
A $1.2$ kb DNA fragment was cloned into $\textit{Bam}$HI and $\textit{Eco}$RI sites located on a $2.8$ kb cloning vector. The $\textit{Bam}$HI and $\textit{Eco}$RI sites are adjacent to each other on the vector backbone. The vector contains an $\textit{XhoI}$ site located $300$ ... to: $2800$, $700$ and $500$ $2800$, $700$ and $800$ $2500$, $700$ and $800$ $2500$, $1200$ and $300$

A $1.2$ kb DNA fragment was cloned into $\textit{Bam}$HI and $\textit{Eco}$RI sites located on a $2.8$ kb cloning vector. The $\textit{Bam}$HI and $\textit{Eco}$RI sites ...

Milicevic3306

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Milicevic3306 asked Mar 26, 2018

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52

GATE2020: 21
The number of molecules of a nucleotide of molecular weight $300\:g$/mol present in $10$ picomoles is ____________ $\times10^{12}$ (round off to $2$ decimal places).

The number of molecules of a nucleotide of molecular weight $300\:g$/mol present in $10$ picomoles is ____________ $\times10^{12}$ (round off to $2$ decimal places).

soujanyareddy13

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soujanyareddy13 asked Nov 3, 2020

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53

GATE2020: 45
A batch reactor is inoculated with $1 \:g/L$ biomass. Under these conditions, cells exhibit a lag phase of $30$ min. If the specific growth rate in the log phase is $0.00417\;min^{-1}$, the time taken for the biomass to increase to $8 \:g/L$ is ________________ min (round off to $2$ decimal places).

A batch reactor is inoculated with $1 \:g/L$ biomass. Under these conditions, cells exhibit a lag phase of $30$ min. If the specific growth rate in the log phase is $0.00...

soujanyareddy13

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54

GATE2019: 43
Which of the following statements is $ALWAYS\; CORRECT$ about an ideal chemostat? Substrate concentration inside the chemostat is equal to that in the exit stream Optimal dilution rate is lower than critical dilution rate Biomass concentration increases with increase in dilution rate Cell recirculation facilitates ... $Q$ only $P, R$ and $S$ only $P$ and $S$ only $P, Q$ and $S$ only

Which of the following statements is $ALWAYS\; CORRECT$ about an ideal chemostat?Substrate concentration inside the chemostat is equal to that in the exit streamOptimal d...

soujanyareddy13

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soujanyareddy13 asked Nov 3, 2020

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55

GATE BT 2023 | Question: 46
If $1000 \mathrm{bp}$ of a double-helical DNA weighs $1 \times 10^{-18} \mathrm{gm}$ and distance between two $\mathrm{bp}$ is $0.34 \mathrm{~nm}$, the total amount of DNA (in mg, rounded off to one decimal place) required to stretch from Earth to Moon (assuming the distance between Earth and Moon to be $3,74,000 \mathrm{~km}$ ) is ______________.

If $1000 \mathrm{bp}$ of a double-helical DNA weighs $1 \times 10^{-18} \mathrm{gm}$ and distance between two $\mathrm{bp}$ is $0.34 \mathrm{~nm}$, the total amount of DN...

admin

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admin asked May 21, 2023

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56

GATE BT 2023 | Question: 51
Ten playing cards numbered $1,2,3, \ldots, 10$ are placed face down on a table. One card is drawn at random, its number recorded, and then replaced face down. A card is drawn again at random. The probability that the number on the second draw is greater than the number on the first draw (rounded off to two decimal places) is ___________.

Ten playing cards numbered $1,2,3, \ldots, 10$ are placed face down on a table. One card is drawn at random, its number recorded, and then replaced face down. A card is d...

admin

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admin asked May 21, 2023

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2 answers

57

GATE BT 2023 | GA Question: 1
"You are delaying the completion of the task. Send ____________ contributions at the earliest." you are your you're yore

"You are delaying the completion of the task. Send ____________ contributions at the earliest."you areyouryou'reyore

admin

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admin asked May 21, 2023

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58

GATE2016-14
Which one of the following statements is NOT true? In competitive inhibition, substrate and inhibitor compete for the same active site of an enzyme Addition of a large amount of substrate to an enzyme cannot overcome uncompetitive inhibition A transition ... non-competitive inhibition, $K_m$ of an enzyme for its substrate remains constant as the concentration of the inhibitor increases

Which one of the following statements is NOT true?In competitive inhibition, substrate and inhibitor compete for the same active site of an enzymeAddition of a large amou...

Milicevic3306

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Milicevic3306 asked Mar 26, 2018

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59

GATE BT 2023 | Question: 53
An evaporator is insulated using glass wool material of $0.15 \mathrm{~m}$ thickness. The inner most surface and the outer surface of the insulation are at $700{ }^{\circ} \mathrm{C}$ and $80^{\circ} \mathrm{C}$, respectively. The mean thermal ... in $W$ ) through $1.2 \mathrm{~m}^2$ of the evaporator wall surface (rounded off to the nearest integer) is ______________.

An evaporator is insulated using glass wool material of $0.15 \mathrm{~m}$ thickness. The inner most surface and the outer surface of the insulation are at $700{ }^{\circ...

admin

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admin asked May 21, 2023

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60

GATE BT 2023 | Question: 38
E. coli is cultivated in a chemostat operated at a dilution rate of $0.2 h^{-1}$. The values of biomass yield due to oxygen consumption and the steady state biomass concentration are $0.2 g \cdot g^{-1}$ and $10 g \cdot L^{-1}$, respectively. The oxygen transfer rate (in $g \cdot L^{-1} \cdot h^{-1}$) is ______________.

E. coli is cultivated in a chemostat operated at a dilution rate of $0.2 h^{-1}$. The values of biomass yield due to oxygen consumption and the steady state biomass conce...

admin

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admin asked May 21, 2023

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61

GATE BT 2023 | Question: 44
An enzyme (E) catalyzes the biochemical reaction $A \rightarrow B$ with $k_{c a t}$ equal to $500 s^{-1}$. If the initial reaction velocity $\left(V_{0}\right)$ is $10 \mu M . s^{-1}$ at the total enzyme concentration $\left[E_{t}\right]$ of $30 n M$ and substrate concentration $[\mathrm{A}]$ of $40 \mu M$, the value of $K_{m}($ in $\mu M)$ is ______________.

An enzyme (E) catalyzes the biochemical reaction $A \rightarrow B$ with $k_{c a t}$ equal to $500 s^{-1}$. If the initial reaction velocity $\left(V_{0}\right)$ is $10 \m...

admin

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admin asked May 21, 2023

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62

GATE BT 2023 | Question: 18
Which one of the following methods CANNOT be used to determine the secondary structure content of a protein? Circular dichroism spectroscopy Fourier transform infrared spectroscopy Mass spectrometry X-ray crystallography

Which one of the following methods CANNOT be used to determine the secondary structure content of a protein?Circular dichroism spectroscopyFourier transform infrared spec...

admin

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admin asked May 21, 2023

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63

GATE2016-47
$\textit{Saccharomyces cerevisiae}$ is cultured in a chemostat (continuous fermentation) at a dilution rate of $0.5 \: h^{-1}$. The feed substrate concentration is $10 \: g \cdot L^{-1}$. The biomass concentration in the chemostat at steady state will be _________ $g \cdot L^{-1}$. ... $K_s = 0.3 \: g \cdot L^{-1}$ and $s$ is substrate concentration $(g \cdot L^{-1})$.

$\textit{Saccharomyces cerevisiae}$ is cultured in a chemostat (continuous fermentation) at a dilution rate of $0.5 \: h^{-1}$. The feed substrate concentration is $10 \:...

Milicevic3306

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Milicevic3306 asked Mar 26, 2018

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64

GATE BT 2023 | Question: 7
Dendritic cells are involved in cross-presentation of antigens. Which of the following protein(s) is(are) required for cross-presentation? P. Basic leucine zipper ATF-like transcription factor $3$ (BATF3) Q. Membrane associated ring-CH-type finger $1$ (MARCH-1) R. Solute carrier ... $\mathrm{R}$ only $\mathrm{P}, \mathrm{Q}$ and $\mathrm{R}$ only $\text{S}$ only

Dendritic cells are involved in cross-presentation of antigens. Which of the following protein(s) is(are) required for cross-presentation?P. Basic leucine zipper ATF-like...

admin

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admin asked May 21, 2023

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65

GATE BT 2021 | Question: 48
In a random mating population, $Y$ and $y$ are dominant and recessive alleles, respectively. If the frequency of $Y$ allele in both sperm and egg is $0.70$, then the frequency $Y/y$ heterozygotes (rounded off to two decimal places) is ___________.

In a random mating population, $Y$ and $y$ are dominant and recessive alleles, respectively. If the frequency of $Y$ allele in both sperm and egg is $0.70$, then the freq...

go_editor

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go_editor asked Mar 1, 2021

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66

GATE BT 2022 | Question: 48
The amount of biomass in a reactor at the end of the batch process is $50 \; \text{g}.$ Fed-batch operation is initiated by feeding the substrate solution at a constant rate of $1 \; \text{L h}^{-1}.$ The concentration of substrate in the feed is ... system is at quasi-steady state, the maximum amount of biomass after $5 \; \text{h}$ of feeding is __________ $\text{g}.$

The amount of biomass in a reactor at the end of the batch process is $50 \; \text{g}.$ Fed-batch operation is initiated by feeding the substrate solution at a constant r...

Lakshman Bhaiya

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Lakshman Bhaiya asked Mar 20, 2022

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67

GATE BT 2023 | Question: 54
A proportional controller is used to control the temperature of an autoclave from $60^{\circ} \mathrm{C}$ to $130^{\circ} \mathrm{C}$. If the proportional band setting of the controller is $25 \%$, the proportional gain value is ___________.

A proportional controller is used to control the temperature of an autoclave from $60^{\circ} \mathrm{C}$ to $130^{\circ} \mathrm{C}$. If the proportional band setting of...

admin

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admin asked May 21, 2023

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68

GATE2016-46
The empirical formula for biomass of an unknown organism is $CH_{1.8}O_{0.5}N_{0.2}$. To grow this organism, ethanol $(C_2H_5OH)$ and ammonia are used as carbon and nitrogen sources, respectively. Assume no product formation other than biomass. To produce $1$ mole of biomass from $1$ mole of ethanol, the number of moles of oxygen required will be __________

The empirical formula for biomass of an unknown organism is $CH_{1.8}O_{0.5}N_{0.2}$. To grow this organism, ethanol $(C_2H_5OH)$ and ammonia are used as carbon and nitro...

Milicevic3306

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Milicevic3306 asked Mar 26, 2018

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69

GATE BT 2022 | Question: 25
For a pure species, the slope of the melting line $\frac{dP}{dT} \; \text{at} \; -2 \; ^{\circ} \textrm{C} \; \text{is} -5.0665 \times 10^{6} \text{Pa K}^{-1}.$ ... $\text{J} \; \text{mol}^{-1}.$

For a pure species, the slope of the melting line$$\frac{dP}{dT} \; \text{at} \; -2 \; ^{\circ} \textrm{C} \; \text{is} -5.0665 \times 10^{6} \text{Pa K}^{-1}.$$The diff...

Lakshman Bhaiya

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Lakshman Bhaiya asked Mar 20, 2022

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70

GATE2012-30
A single base pair of DNA weighs $1.1 \times 10^{-21}$ grams. How many picomoles of a plasmid vector of length $2750$ bp are contained in 1 $\mu$g of purified DNA? $0.30$ $0.55$ $0.25$ $0.91$

A single base pair of DNA weighs $1.1 \times 10^{-21}$ grams. How many picomoles of a plasmid vector of length $2750$ bp are contained in 1 $\mu$g of purified DNA?$0.30$$...

Milicevic3306

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Milicevic3306 asked Mar 25, 2018

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71

GATE BT 2021 | Question: 51
A batch cultivation of $\textit{E. coli}$ follows zeroth order Monod's growth kinetics. The cell growth is terminated when the residual dissolved oxygen concentration attains $10\%$ of its saturation value and oxygen mass transfer coefficient $(k_La)$ reaches its ... (rounded off to two decimal places) at the end of the batch cultivation is __________

A batch cultivation of $\textit{E. coli}$ follows zeroth order Monod’s growth kinetics. The cell growth is terminated when the residual dissolved oxygen concentration a...

go_editor

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go_editor asked Mar 1, 2021

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72

GATE BT 2023 | Question: 43
The following schematic diagram shows a chemostat with cell recycle where $\mathrm{F}_0$ and $\mathrm{F}_{\mathrm{r}}$ are the volumetric flow rates (in $L \cdot h^{-1}$ ) of feed and recycle streams, respectively. $\mathrm{X}_1, \mathrm{X}_0$ ... $\mathrm{X}$ (in $g \cdot L^{-1}$, rounded off to one decimal place) is ________________.

The following schematic diagram shows a chemostat with cell recyclewhere $\mathrm{F}_0$ and $\mathrm{F}_{\mathrm{r}}$ are the volumetric flow rates (in $L \cdot h^{-1}$ )...

admin

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admin asked May 21, 2023

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73

GATE2016-43
The molar extinction coeffcients of Trp and Tyr at $280$ nm are $5690$ and $1280 \: M^{-1} \cdot cm^{-1}$, respectively. The polypeptide chain of yeast alcohol dehydrogenase ($37$ kDa) contains $5$ Trp and $14$ Tyr residues. ... pathlength will be _________ (Assume that the molar extinction coefficient values for Trp and Tyr apply to these amino acids inthe yeast alcohol dehydrogenase).

The molar extinction coeffcients of Trp and Tyr at $280$ nm are $5690$ and $1280 \: M^{-1} \cdot cm^{-1}$, respectively. The polypeptide chain of yeast alcohol dehydrogen...

Milicevic3306

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Milicevic3306 asked Mar 26, 2018

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74

GATE BT 2023 | GA Question: 6
Human beings are one among many creatures that inhabit an imagined world. In this imagined world, some creatures are cruel. If in this imagined world, it is given that the statement "Some human beings are not cruel creatures" is FALSE, then which of the following set of statement( ... $\text{(i)}$ and $\text{(ii)}$ $\text{(i), (ii)}$ and $\text{(iii)}$

Human beings are one among many creatures that inhabit an imagined world. In this imagined world, some creatures are cruel. If in this imagined world, it is given that th...

admin

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admin asked May 21, 2023

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75

GATE BT 2023 | Question: 13
In hybridoma technology, which one of the following enzymes is absent in the myeloma cells that are used for monoclonal antibody production? Hypoxanthine-guanine phosphoribosyltransferase Alanine aminotransferase Triose phosphate isomerase Glycosyltransferase

In hybridoma technology, which one of the following enzymes is absent in the myeloma cells that are used for monoclonal antibody production?Hypoxanthine-guanine phosphori...

admin

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admin asked May 21, 2023

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76

GATE2016-45
Analysis of a hexapeptide using enzymatic cleavage reveals the following result: Amino acid composition of the peptide is: $2R, A, V, S, Y$ Trypsin digestion yields two fragments and the compositions are: $(R A,V)$ and $(R, S, Y)$ Chymotrypsin digestion yields ... peptide. The correct amino acid sequence of the peptide is $\text{RSYRVA}$ $\text{AVRYSR}$ $\text{SRYVAR}$ $\text{SVRRYA}$

Analysis of a hexapeptide using enzymatic cleavage reveals the following result:Amino acid composition of the peptide is: $2R, A, V, S, Y$Trypsin digestion yields two fra...

Milicevic3306

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77

GATE2020: 16
A microorganism isolated from a salt-rich (salt concentration $\sim2$ $M$) lake was found to possess diglycerol tetraethers, with polyisoprenoid alcohol side chains, as the major lipid component of its cell membrane. The isolated organism is a planctomycete a cyanobacteria a unicellular amoeba an archaea

A microorganism isolated from a salt-rich (salt concentration $\sim2$ $M$) lake was found to possess diglycerol tetraethers, with polyisoprenoid alcohol side chains, as t...

soujanyareddy13

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soujanyareddy13 asked Nov 3, 2020

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78

GATE2015-56
Assuming random distribution of nucleotides, the average number of fragments generated upon digestion of a circular DNA of size $4.3 \times 10^5$ bp with $\textit{Alu}I (5 ' – AG \downarrow CT-3' )$ is _________$ \times 10^3$

Assuming random distribution of nucleotides, the average number of fragments generated upon digestion of a circular DNA of size $4.3 \times 10^5$ bp with $\textit{Alu}I (...

Milicevic3306

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Milicevic3306 asked Mar 26, 2018

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79

GATE BT 2021 | Question: 25
The enzyme $\alpha$-amylase used in starch hydrolysis has an affinity constant $(K_m)$ value of $0.005 \: M$. To achieve one-fourth of the maximum rate of hydrolysis, the required starch concentration $mM$ (rounded off to two decimal places) is ____________

The enzyme $\alpha$-amylase used in starch hydrolysis has an affinity constant $(K_m)$ value of $0.005 \: M$. To achieve one-fourth of the maximum rate of hydrolysis, the...

go_editor

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go_editor asked Mar 1, 2021

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80

GATE2012-51
Answer the question based on the information given below: The purification data for an enzyme is given below: ... $\text{P-3.4, Q-2.4, R-1.5, S-0.1}$ $\text{P-100, Q-71, R-44, S-29}$

Answer the question based on the information given below:The purification data for an enzyme is given below:$\begin{array}{|l|l|l|l|l|l|} \hline & \text{Step} & \text{Vol...

Milicevic3306

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Milicevic3306 asked Mar 25, 2018