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481
GATE2015-25
Which one of the following is INCORRECT about a typical apoptotic cell? Phosphatidylserine is presented on the outer cell surface Cytochrome c is released from mitochondria Mitochondrial membrane potential does not change Annexin-V binds to the cell surface
Which one of the following is INCORRECT about a typical apoptotic cell?Phosphatidylserine is presented on the outer cell surfaceCytochrome c is released from mitochondria...
Milicevic3306
7.9k
points
Milicevic3306
asked
Mar 26, 2018
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482
GATE2015-64
Biomass is being produced in a continuous stirred tank bioreactor of $750$ L capacity. The sterile feed containing $8 \: g \cdot L^{-1}$ glucose as substrate was fed at a flow rate of $150 \: L \cdot h^{-1}$ ... Determine the cell productivity $(g \cdot L^{-1} \cdot h^{-1})$ at steady state. $0.85$ $0.65$ $0.45$ $0.25$
Biomass is being produced in a continuous stirred tank bioreactor of $750$ L capacity. The sterile feed containing $8 \: g \cdot L^{-1}$ glucose as substrate was fed at a...
Milicevic3306
7.9k
points
Milicevic3306
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Mar 26, 2018
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gate2015
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483
GATE2015-36
An isolated population on an island has the following genotypic frequencies: $\begin{array}{|c|c|c|c|} \hline \textrm{Genotype} & AA & Aa & aa \\ \hline \textrm{Frequency} & 0.3 & 0.4 & 0.3 \\ \hline \end{array}$ Assuming that there are only two alleles ($A$ and $a$) for the gene, the genotypic frequency of $AA$ in the next generation will be __________
An isolated population on an island has the following genotypic frequencies: $$\begin{array}{|c|c|c|c|} \hline \textrm{Genotype} & AA & Aa & aa \\ \hline \textrm{Frequenc...
Milicevic3306
7.9k
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Mar 26, 2018
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gate2015
numerical-answers
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484
GATE2017-31
Match the organisms in $\text{Column I}$ with the characteristics in $\text{Column II}$ ... $\text{P-2, Q-1, R-4, S-3}$ $\text{P-4, Q-1, R-2, S-3}$
Match the organisms in $\text{Column I}$ with the characteristics in $\text{Column II}$:$\begin{array}{|l|l|l|l|} \hline & \textbf{Column I} && \textbf{Column II} \\ \hli...
Milicevic3306
7.9k
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gate2017
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485
GATE2014-51
If protoplasts are placed in distilled water, they swell and burst as a result of endosmosis. The plot representing the kinetics of burst is
If protoplasts are placed in distilled water, they swell and burst as a result of endosmosis.The plot representing the kinetics of burst is
Milicevic3306
7.9k
points
Milicevic3306
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Mar 25, 2018
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gate2014
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486
GATE2014-46
Match the immunoglobulin class in $\text{Group I}$ with its properties in $\text{Group II}$ ... $\text{P-4, Q-3, R-1, S-2}$ $\text{P-1, Q-4, R-3, S-2}$
Match the immunoglobulin class in $\text{Group I}$ with its properties in $\text{Group II}$$\begin{array}{|l|l|l|l|} \hline & \textbf{Group I} && \textbf{Group II} \\ \hl...
Milicevic3306
7.9k
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Milicevic3306
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Mar 25, 2018
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gate2014
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487
GATE2015-24
Production of monoclonal antibodies by hybridoma technology requires splenocytes osteocytes hepatocytes thymocytes
Production of monoclonal antibodies by hybridoma technology requiressplenocytesosteocyteshepatocytesthymocytes
Milicevic3306
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488
GATE2017-32
Which one of the following amino acids has three ionizable groups? Glycine Leucine Valine Lysine
Which one of the following amino acids has three ionizable groups?GlycineLeucineValineLysine
Milicevic3306
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gate2017
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489
GATE2017-24
A bacterium has a genome of size $6$ million base pairs. If the average rate of DNA synthesis is $1000$ base pairs/second, the time taken (in minutes) for replication of the genome will be ________.
A bacterium has a genome of size $6$ million base pairs. If the average rate of DNA synthesis is $1000$ base pairs/second, the time taken (in minutes) for replication of ...
Milicevic3306
7.9k
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490
GATE2015-53
Consider the following multiple sequence alignment of four DNA sequences. $\begin{array}{cccc} A & C & T & A \\ A & C & T & G \\ A & G & T & C \\ A & G & C & T \end{array}$Shannon's entropy of the above alignment is ___________
Consider the following multiple sequence alignment of four DNA sequences.$$\begin{array}{cccc} A & C & T & A \\ A & C & T & G \\ A & G & T & C \\ A & G & C & T \end{arra...
Milicevic3306
7.9k
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gate2015
numerical-answers
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491
GATE2015-46
Plasmid DNA $(0.5 \: \mu g)$ containing an ampicillin resistance marker was added to $200 \: \mu l$ of competent cells. The transformed competent cells were diluted $10,000$ times, out of which, $50 \: \mu l$ was plated on agar plates containing ampicillin. A total of $35$ colonies were obtained. The transformation efficiency is _______$\times 10^6 \: \text{ cfu} \cdot \mu g^{-1}$
Plasmid DNA $(0.5 \: \mu g)$ containing an ampicillin resistance marker was added to $200 \: \mu l$ of competent cells. The transformed competent cells were diluted $10,0...
Milicevic3306
7.9k
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gate2015
numerical-answers
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492
GATE2017-55
Which of the following conditions promote the development of human autoimmune disorders? Inability to eliminate self-reactive lymphocytes Generation of auto-antibodies Ability to eliminate self-reactive T-cells Induction of regulatory T-cells in the thymus P,R Q,S P,Q R,S
Which of the following conditions promote the development of human autoimmune disorders?Inability to eliminate self-reactive lymphocytesGeneration of auto-antibodiesAbili...
Milicevic3306
7.9k
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493
GATE2017-34
The concentration (in micromolar) of NADH in a solution with $A_{340}=0.50$ is _________ Given data: Path length $=1$ cm; Molar extinction coefficient of NADH $\varepsilon_{340}= 6220 M^{-1}cm^{-1}$
The concentration (in micromolar) of NADH in a solution with $A_{340}=0.50$ is _________Given data: Path length $=1$ cm; Molar extinction coefficient of NADH $\varepsilon...
Milicevic3306
7.9k
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Mar 26, 2018
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gate2017
numerical-answers
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494
GATE2017-20
A polymerase chain reaction (PCR) was set up with the following reagents: DNA template, Taq polymerase, buffer, dNTPs, and Mg$^{2+}$. Which one of the following is missing in the reaction mixture? Helicase Single-stranded binding proteins Primers Reverse transciptase
A polymerase chain reaction (PCR) was set up with the following reagents: DNA template, Taq polymerase, buffer, dNTPs, and Mg$^{2+}$. Which one of the following is missin...
Milicevic3306
7.9k
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495
GATE2017-45
A pedigree of an inheritable disease is shown below. This inheritable disease is $X$-linked dominant $X$-linked recessive or Y-linked only $Y$-linked only $X$-linked recessive
A pedigree of an inheritable disease is shown below.This inheritable disease is$X$-linked dominant$X$-linked recessive or Y-linkedonly $Y$-linkedonly $X$-linked recessive...
Milicevic3306
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496
GATE2013-55
What is the holding period (min) at a $K$ value of $\text{3.36min}^{-1}?$ $10.6$ $9.5$ $8.4$ $7.2$
What is the holding period (min) at a $K$ value of $\text{3.36min}^{-1}?$$10.6$$9.5$$8.4$$7.2$
Milicevic3306
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497
GATE2015-30
In DNA sequencing reactions using the chain termination method, the ratio of ddNTPs to dNTPs should be $0$ $< 1$ $1$ $>1$
In DNA sequencing reactions using the chain termination method, the ratio of ddNTPs to dNTPs should be$0$$< 1$$1$$>1$
Milicevic3306
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498
GATE2015-48
$\textit{Saccharomyces cerevisiae}$ produces ethanol by fermentation. The theoretical yield of ethanol from $2.5$ g of glucose is _________ g.
$\textit{Saccharomyces cerevisiae}$ produces ethanol by fermentation. The theoretical yield of ethanol from $2.5$ g of glucose is _________ g.
Milicevic3306
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gate2015
numerical-answers
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499
GATE2014-30
A T- ask is seeded with $10^5$ anchorage-dependent cells. The available area of the T- ask is $25\: cm^2$ and the volume of the medium is $25\: ml$. Assume that the cells are rectangles of size $5\: \mu m \times 2\: \mu m$. If the cells grow to monolayer confluence after $50\: h$, the growth rate in number of $cells/(cm^2 .h)$ is _________$ \times10^5$
A T- ask is seeded with $10^5$ anchorage-dependent cells. The available area of the T- ask is $25\: cm^2$ and the volume of the medium is $25\: ml$. Assume that the cells...
Milicevic3306
7.9k
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Milicevic3306
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Mar 25, 2018
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gate2014
numerical-answers
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500
GATE2014-22
The algorithm for BLAST is based on Dynamic Programming Hidden Markov Model k-tuple analysis Neural Network
The algorithm for BLAST is based onDynamic ProgrammingHidden Markov Modelk-tuple analysisNeural Network
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Mar 25, 2018
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gate2014
models
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501
GATE2013-21
A callus of $\text{5 g}$ dry weight was inoculated on semi-solid medium for growth. The dry weight of the callus was found to increase by $1.5$ fold after $10$ days of inoculation. The growth index of the culture is ____________.
A callus of $\text{5 g}$ dry weight was inoculated on semi-solid medium for growth. The dry weight of the callus was found to increase by $1.5$ fold after $10$ days of in...
Milicevic3306
7.9k
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Mar 25, 2018
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gate2013
numerical-answers
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502
GATE2013-24
Calculate the percentage sequence identity for the pairwise alignment given below. ______________ $\text{HELLO-}$ $\text{YELLOW}$
Calculate the percentage sequence identity for the pairwise alignment given below. ______________$\text{HELLO-}$$\text{YELLOW}$
Milicevic3306
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Milicevic3306
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Mar 25, 2018
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gate2013
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503
GATE2014-41
In a mouse genome, the numbers of functional $V\alpha, J\alpha, V\beta, D\beta, J\beta$ gene segments are $\text{79, 38, 21, 2 and 11,}$ respectively. The total number of possible combinations for $\alpha\beta$ $T$ cell receptors are ___________$\times10^6 .$
In a mouse genome, the numbers of functional $V\alpha, J\alpha, V\beta, D\beta, J\beta$ gene segments are $\text{79, 38, 21, 2 and 11,}$ respectively. The total number of...
Milicevic3306
7.9k
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Milicevic3306
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Mar 25, 2018
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gate2014
numerical-answers
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504
GATE2014-23
The statistical frequency of the occurrence of a particular restriction enzyme cleavage site that is $6$ bases long can be estimated to be once every $24$ bases once every $256$ bases once every $1024$bases once every $4096$ bases
The statistical frequency of the occurrence of a particular restriction enzyme cleavage site that is $6$ bases long can be estimated to beonce every $24$ basesonce every ...
Milicevic3306
7.9k
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Milicevic3306
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Mar 25, 2018
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gate2014
numerical-estimation
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505
GATE2012-18
Identify the statement that is NOT applicable to an enzyme catalyzed reaction. Enzyme catalysis involves propinquity effects The binding of substrate to the active site causes a strain in the substrate Enzymes do not accelerate the rate of reverse reaction Enzyme catalysis involves acid-base chemistry
Identify the statement that is NOT applicable to an enzyme catalyzed reaction.Enzyme catalysis involves propinquity effectsThe binding of substrate to the active site cau...
Milicevic3306
7.9k
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Mar 25, 2018
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gate2012
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506
GATE2014-38
Match the following photoreceptors with their prosthetic groups and spectral specificity: ... $\text{P-3-a, Q-1-a, R-1-c}$ $\text{P-2-c, Q-1-c, R-1-a}$
Match the following photoreceptors with their prosthetic groups and spectral specificity:$\begin{array}{|l|l|l|} \hline \textbf{Photoreceptor} & \textbf{Moiety that absor...
Milicevic3306
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Mar 25, 2018
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507
GATE2013-54
During sterilization of a fermentation medium in a given bioreactor $\nabla_{heaing}=12.56, \nabla_{cooling}= 7.48$ and the total value of $\nabla$ required for whole sterilization process is $52$, where $\nabla$ is the design criteria. What is the value of $\nabla_{holding}$? $31.96$ $42.32$ $52.43$ $61.18$
During sterilization of a fermentation medium in a given bioreactor $\nabla_{heaing}=12.56, \nabla_{cooling}= 7.48$ and the total value of $\nabla$ required for whole ste...
Milicevic3306
7.9k
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Mar 25, 2018
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508
GATE2013-49
A solution was prepared by dissolving $\text{100 mg}$ of protein $X$ in $\text{100 ml}$ of water. Molecular weight of protein $X$ is $\text{15,000 Da};$ Avogadro’s number = $6.022\times 10^{23}$. The number of molecules present in this solution is $40.15\times10^{19}$ $6.023\times10^{19}$ $4.015\times10^{19}$ $0.08\times10^{19}$
A solution was prepared by dissolving $\text{100 mg}$ of protein $X$ in $\text{100 ml}$ of water. Molecular weight of protein $X$ is $\text{15,000 Da};$ Avogadro’s numb...
Milicevic3306
7.9k
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Mar 25, 2018
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509
GATE2014-48
The length of the minimum unique stretch of DNA sequence that can be found only once in a $3$ billion base pairs long genome is $14$ $15$ $16$ $18$
The length of the minimum unique stretch of DNA sequence that can be found only once in a $3$ billion base pairs long genome is$14$$15$$16$$18$
Milicevic3306
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510
GATE2014-49
Lysine is being produced in a lab-scale reactor by a threonine auxotroph. After 2 weeks of operation it was observed that the concentration of lysine in the reactor was gradually decreasing.Microbiological assays of reactor samples showed ... lysine concentration may be attributed to accumulation of ethanol growth of revertants production of citric acid unutilized phosphoenol pyruvate
Lysine is being produced in a lab-scale reactor by a threonine auxotroph. After 2 weeks of operation it was observed that the concentration of lysine in the reactor was g...
Milicevic3306
7.9k
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Milicevic3306
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Mar 25, 2018
Others
gate2014
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511
GATE2012-31
Match the terms in $\textbf{Group I}$ with the ploidy in $\textbf{Group II}$ ... $\text{P-4, Q-3, R-1, S-2}$ $\text{P-2, Q-3, R-4, S-1}$ $\text{P-l, Q-4, R-3, S-2}$
Match the terms in $\textbf{Group I}$ with the ploidy in $\textbf{Group II}$.$\begin{array}{|C|l|C|l|} \hline &\textbf{Group I} & {} &\textbf{Group II} \\\hline P. &\text...
Milicevic3306
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gate2012
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512
GATE2014-6
The unit for specific substrate consumption rate in a growing culture is $\frac{g}{L-h}$ $\frac{g}{h}$ $\frac{g}{g-h}$ $\frac{gmoles}{L-h}$
The unit for specific substrate consumption rate in a growing culture is$\frac{g}{L-h}$$\frac{g}{h}$$\frac{g}{g-h}$$\frac{gmoles}{L-h}$
Milicevic3306
7.9k
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Milicevic3306
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Mar 25, 2018
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gate2014
numerical-estimation
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513
GATE2013-53
A DNA fragment of $5000bp$ needs to be isolated from E.coli (genome size $4\times10^3kb$) genomic library. The number of clones to represent this fragment in genomic library with a probability of $95\%$ are $5.9\times10^3$ $4.5\times10^3$ $3.6\times10^3$ $2.4\times10^3$
A DNA fragment of $5000bp$ needs to be isolated from E.coli (genome size $4\times10^3kb$) genomic library.The number of clones to represent this fragment in genomic libra...
Milicevic3306
7.9k
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Milicevic3306
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Mar 25, 2018
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514
GATE2014-43
In an affine gap penalty model, if the gap opening penalty $-20$, gap extension penalty is $-4$ and gap length is $8$, the gap score is__________.
In an affine gap penalty model, if the gap opening penalty $-20$, gap extension penalty is $-4$ and gap length is $8$, the gap score is__________.
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Milicevic3306
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Mar 25, 2018
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gate2014
numerical-answers
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515
GATE2014-52
Which of the following statements with respect to the orientation of the nitrogenous bases to the pentose sugars, and the puckering of the sugar, is correct? Anti, and 2’-endo in A form DNA Anti, and 2’-endo in B form DNA Syn, and 3’-endo in A form DNA Syn, and 3’-endo in B form DNA
Which of the following statements with respect to the orientation of the nitrogenous bases to the pentose sugars, and the puckering of the sugar, is correct?Anti, and 2�...
Milicevic3306
7.9k
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Milicevic3306
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Mar 25, 2018
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gate2014
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516
GATE2013-36
Determine the correctness or otherwise of the following $\text{Assertion (a) and Reason (r)}$. $\text{Assertion:}$ $\text{UPGMA}$ method produces ultrametric tree. $\text{Reason:}$ Sequence alignment is converted into evolutionary distances in $\text{UPGMA}$ method. Both $(a)$ and $(r)$ ... the correct reason for $(a)$ $(a)$ is true but $(r)$ is false $(a)$ is false but $(r)$ is true
Determine the correctness or otherwise of the following $\text{Assertion (a) and Reason (r)}$.$\text{Assertion:}$ $\text{UPGMA}$ method produces ultrametric tree.$\text{R...
Milicevic3306
7.9k
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Milicevic3306
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Mar 25, 2018
Others
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517
GATE2014-44
For their efficient translation, eubacterial $mRNAs$ possess a Shine-Dalgarno sequence for its recognition by an anti-Shine-Dalgarno sequence $(ASD)$ in the ribosomes. The correct statement is $ASD$ is present in $\text{5S rRNA}$ $ASD$ is present in $\text{23S rRNA}$ ... $\text{23S rRNA}$ upon docking of the $50S$ subunit on the $30S$ subunit of the ribosomes
For their efficient translation, eubacterial $mRNAs$ possess a Shine-Dalgarno sequence for its recognition by an anti-Shine-Dalgarno sequence $(ASD)$ in the ribosomes. Th...
Milicevic3306
7.9k
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Milicevic3306
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Mar 25, 2018
Others
gate2014
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518
GATE2014-34
The plasmid DNA was subjected to restriction digestion using the enzyme $\textit{EcoRI}$ and analysed on an agarose gel. Assuming digestion has worked (the enzyme was active), match the identity of the DNA bands shown in the image in $\text{Group I}$ with their identity in $\text{Group II}$ ... $\text{P-4, Q-3, R-1, S-2}$ $\text{P-4, Q-1, R-2, S-3}$
The plasmid DNA was subjected to restriction digestion using the enzyme $\textit{EcoRI}$ and analysed on an agarose gel. Assuming digestion has worked (the enzyme was act...
Milicevic3306
7.9k
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Milicevic3306
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Mar 25, 2018
Others
gate2014
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519
GATE2014-47
In a genetic cross between the genotypes $WWXX$ and $\text{wwxx}$, the following phenotypic distributions were observed among the $F_2$ progeny: $\text{WX, 562; wx, 158; WX, 38; and wX, 42}$. Likewise, a cross between $XXYY$ and $\text{xxyy}$ ... units Relative position: $\text{X-W-Y}$ Distances: $\text{X-W = 5}$ map units, $\text{W-Y = 10}$ map units
In a genetic cross between the genotypes $WWXX$ and $\text{wwxx}$, the following phenotypic distributions were observed among the $F_2$ progeny: $\text{WX, 562; wx, 158; ...
Milicevic3306
7.9k
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Milicevic3306
asked
Mar 25, 2018
Others
gate2014
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0
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520
GATE2014-50
If a plant is shifted to cold temperature, which of the following changes would take place in its membrane? Ratio of unsaturated to saturated fatty acids would increase Ratio of unsaturated to saturated fatty acids would decrease Absolute amount of both fatty acids would increase keeping the ratio same Absolute amount of both fatty acids would remain unchanged
If a plant is shifted to cold temperature, which of the following changes would take place in its membrane?Ratio of unsaturated to saturated fatty acids would increaseRat...
Milicevic3306
7.9k
points
Milicevic3306
asked
Mar 25, 2018
Others
gate2014
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