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For their efficient translation, eubacterial $mRNAs$ possess a Shine-Dalgarno sequence for its recognition by an anti-Shine-Dalgarno sequence $(ASD)$ in the ribosomes. The correct statement is

  1. $ASD$ is present in $\text{5S rRNA}$
  2. $ASD$ is present in $\text{23S rRNA}$
  3. $ASD$ is present in $\text{16S rRNA}$
  4. $ASD$ is formed by the interaction of the $\text{16S rRNA}$ with the $\text{23S rRNA}$ upon docking of the $50S$ subunit on the $30S$ subunit of the ribosomes

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