Questions without a selected answer in Bioreaction Engineering

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The correct sequence of the four steps involved in anaerobic production of biogas from complex organics is:Hydrolysis → Acidogenesis → Acetogenesis → MethanogenesisMethan...
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A microbial culture is being grown in a bioreactor with continuous aeration. The dissolved oxygen probe provided with the system shows a reading of zero. In this context,...
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In an aerobic fermentation with air sparging, which of the following options can be used to increase the volumetric mass transfer coefficient for oxygen transfer?Using pu...
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For an ideal plug flow reactor, which of the following statements is/are true?It can be approximated by a large number of ideal continuous stirred tank reactors connected...
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A microbial culture (following Monod model for growth) has a maximum specific growth rate of $0.1 \mathrm{~h}^{-1}$, Monod constant of $1 \mathrm{mg} \mathrm{L}^{-1}$ and...
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A $\text{CSTR}$ with a volume of $100 \mathrm{~m}^{3}$ is operated in cell recycle mode. At a volumetric flow rate of $10 \mathrm{~m}^{3}$ day ${ }^{-1}$ and effluent bio...
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A batch sterilizer is being operated at $121^{\circ} \mathrm{C}$ for sterilizing a medium containing microbial cells. Assume that the thermal deactivation of cells is a f...
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A substrate is consumed in a zero order reaction such that its concentration falls from $42 \mathrm{~g} \mathrm{~L}^{-1}$ to $14 \mathrm{~g} \mathrm{~L}^{-1}$ in $4$ hour...
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A microbe that follows Monod growth kinetics on a limiting substrate (maximum specific growth rate of $0.5 \mathrm{~h}^{-1}$ and Monod constant of $0.1 ~\mathrm{mg} \math...
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It is desired to cultivate $150$ mg (dry weight) microbial cells with an empirical formula of $\mathrm{C}_{5} \mathrm{H}_{7} \mathrm{O}_{2} \mathrm{~N}$. If the phosphoru...
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A packed bed bioreactor with length $1 \text{~m}$ and inside diameter $10 \text{~cm}$ has liquid flowing at an interstitial velocity of $1 \mathrm{~cm} \mathrm{~s}^{-1}$....
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​​​​Correctly match the following Product classes with their representative Products.Product classProductP. Biofuel1. CellulaseQ. Bioplastic2. CephalosporinR. Industrial ...
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​​​​Which of the following fluids exhibit(s) non-Newtonian behaviour at $25^{\circ} \mathrm{C}$ ?ToothpasteMercuryBrineBlood plasma
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​​​​For a specific bioreactor configuration, the power requirement for a Rushton-turbine impeller agitating an unaerated Newtonian fluid in the turbulent regime will bepr...
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​​​​An enzyme immobilised in a porous spherical pellet, catalyses a strongly mass-transfer limited first-order reaction. The effectiveness factor for the immobilised enzy...
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A binary mixture of benzene and toluene under vapour-liquid equilibrium at $80^{\circ} \mathrm{C}$ follows ideal Raoult's law. At this condition, the saturation pressures...
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In a fermentation process, each mole of glucose is converted to biomass $\left(\mathrm{CH}_{1.8} \mathrm{O}_{0.5} \mathrm{~N}_{0.2}\right)$, with a biomass yield coeffici...
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A hot, freshly-sterilised fermentation medium is cooled in a double-pipe heat-exchanger. The medium enters the inner pipe of the exchanger at $95^{\circ} \mathrm{C}$ and ...
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A $2 \: \mathrm{L}$ bioreactor is being operated as a chemostat, at a flow rate of $0.8 \: \mathrm{~L} / \mathrm{h}$ and sterile feed of $10 \: \mathrm{~g} / \mathrm{L}$ ...
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Aqueous two-phase extraction is used to recover α-amylase from a solution. A polypropylene glycol-dextran mixture is added and the solution separates into upper and lower...
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Which one of the following statements is correct about Reynolds Number $\left(N_{R e}\right)$ in a stirred tank bioreactor?$N_{R e}$ is independent of the viscosity of th...
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Under complete cell washout condition in a chemostat with sterile feed, which of the following statements is/are correct?Biomass concentration in the reactor is maximumS...
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Fermentation medium is cooled from $121^{\circ} \mathrm{C}$ to $30{ }^{\circ} \mathrm{C}$ in a double pipe heat exchanger. If cold water is flowing in the counter-current...
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Aspergillus niger is grown in a $10,000$ L stirred batch bioreactor under aerated conditions to produce citric acid. At steady state oxygen transfer conditions, the speci...
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Ethanol is produced in a $10,000$ L stirred bioreactor using an impeller of diameter $1 \mathrm{~m}$. The density and viscosity of fermentation broth are $1000 \mathrm{~k...
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A fermentor is filled with medium at a rate of $1 \mathrm{~L} \mathrm{~min}^{-1}$. A leak develops at the bottom of the fermentor when the medium in the fermentor reaches...
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A fed batch process is running at quasi-steady state with respect to substrate and biomass concentration. At $2 \mathrm{~h}$, the culture volume is $500 \mathrm{~L}$ with...
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Consider scale-up of fungal fermentation from a $20 \mathrm{~L}$ model-type to $20,000 \mathrm{~L}$ prototype stirred tank reactor. The model-type and prototype have the ...
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A batch cultivation of E. coli follows zeroth order Monod’s growth kinetics. The cell growth is terminated when the residual dissolved oxygen concentration attains 10% of...
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The following reaction represents biomass synthesis from hexadecane$\mathrm{C}_{16} \mathrm{H}_{34}+12.5 \mathrm{O}_2+2.13 \mathrm{NH}_3 \rightarrow 10.6 \mathrm{CH}_{1.6...
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E. coli is cultivated in a chemostat operated at a dilution rate of $0.2 h^{-1}$. The values of biomass yield due to oxygen consumption and the steady state biomass conce...
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The following schematic diagram shows a chemostat with cell recyclewhere $\mathrm{F}_0$ and $\mathrm{F}_{\mathrm{r}}$ are the volumetric flow rates (in $L \cdot h^{-1}$ )...
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The values of the consistency index ' $K$ ' and the flow behavior index ' $n$ ' of a dilatant fluid are 0.415 (in CGS units) and $1.23$, respectively. The value of the ap...
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For a double-pipe heat exchanger, the inside and outside heat transfer coefficients are $100$ and $200 \; \text{W m}^{-2} \text{K}^{-1},$ respectively. The thickness and ...
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The overall stoichiometry for an aerobic cell growth is$3 \text{C}_{6} \text{H}_{12} \text{O}_{6} + 2.5 \text{NH}_{3} + \text{O}_{2} \rightarrow 1.5 \text{C}_{a} \text{H}...
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A microorganism is grown in a batch culture using glucose as a carbon source. The apparent growth yield is $0.5 \frac{\text{g biomass}}{\text{g substrate}}.$ The initial ...
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The degree of reduction of lactic acid $(\text{C}_{3} \text{H}_{6} \text{O}_{3})$ is __________.
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The specific growth rate of a yeast having a doubling time of $0.693 \; \text{h}$ (rounded off to nearest integer) is ________ $\text{h}^{-1}.$
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A fermentation broth of density $1000 \; \text{kg m}^{-3}$ and viscosity $10^{-3} \; \text{kg m}^{-1} \text{s}^{-1}$ is mixed in a $100\;\text{L}$ fermenter using a $0.1 ...
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