Questions without a selected answer in General Biology

Cancer cells display a high level of heterogeneity in the expression of TP$53$ protein. Which of the following techniques can be directly used to determine the intratumor...

In nitrogen fixation, to reduce nitrogen to ammonia, which one of the following shows the correct order of electron flow?Ferredoxin → reductase → nitrogenaseNADH → reduct...

For monoclonal antibody production, hypoxanthine $\_\_\_\_$.allows DNA synthesis in the absence of de novo dTMP synthesisallows purine synthesis in cells containing HGPRT...

Which one of the following proteins would elute third in gel filtration chromatography from a mixture of four proteins given below?Insulin $(6 \text{ kDa})$Myoglobin $(17...

Which one of the following antibiotics blocks protein chain elongation by preventing the action of peptidyl transferase?BleomycinRifampicinChloramphenicolTetracycline

The RNA primer synthesized during bacterial DNA replication is removed by $\_\_\_\_$.DNA gyraseDNA Polymerase $\text{I}$PrimaseDNA Polymerase $\text{III}$

Match the carbohydrates in Column $\text{I}$ with the correct glycosidic linkages involved in Column $\text{II}$.\[\begin{array}{|l|l|}\hline\text{Column I} & \text{Colum...

BLAST and its extensions can be used to determine similarity of a query protein sequence with which of the following database(s)?A database of translated nucleotide seque...

You are characterizing a new enzyme isolated and purified in the laboratory. If the maximum velocity of the enzyme is $1800 ~\mu$ moles $\mathrm{L}^{-1} \mathrm{~min}^{-1...

To determine the viable cell count of a bacterial culture, you have plated $50 ~\mu \mathrm{~L}$ of a $100$ -fold diluted sample of the culture on a nutrient agar plate a...

You have purified an enzyme using a series of chromatographic methods. It was observed that a $10 ~\mu \mathrm{g} \mathrm{~mL}^{-1}$ of this purified enzyme converted $10...

Match the immunological terms in $\textbf{Column I}$ with their function/description in the $\textbf{Column II}$\[\begin{array}{|l|l|}\hline\textbf{Column I} & \textbf{Co...

Match the biomolecules in Column $\text{I}$ with their function given in Column $\text{II}$\[\begin{array}{|l|l|}\hline\text{Column I} & \text{Column II} \\\hline\text{P....

By elemental analysis of a biomass sample, it was found that the cells in the given sample are composed of (by weight) $\mathrm{C}=50 \%, \mathrm{H}=8 \%, \mathrm{O}=20 \...

Messenger $\text{RNAs (mRNAs)}$ translate to generate polypeptides and the translation terminates at the $\text{UGA, UAG}$ or $\text{UAA}$ stop codons. The $\text{AGA}$ c...

The RNA sequence below depicts the part of a $330$ nucleotides long $\text{mRNA}$ and it encodes the $\text{C}$-terminal portion of a protein.$5^{\prime}-\ldots \ldots$ $...

Which of the following is/are posttranslational modification(s) involved in epigenetic control of gene expression?Arginine methylationLysine acetylationCytosine methylati...

The electron equivalent per liter (e-eq $\mathrm{L}^{-1}$ ) of acetate ion solution of concentration $10 \mathrm{~g} \mathrm{~L}^{-1}$ is $\_\_\_\_$ $\mathrm{e}^{-} \math...

In the process of generating a clonal mammalian cell line, a single cell was seeded in a well of a cell culture plate. After the first $48$ hours, one of the progeny cell...

​​​​​The diversity in $T$-cell receptors is generated bygene rearrangementssomatic hypermutation of rearranged $V$ regiongene conversionclass switching

​​​​Which one of the following is true for piRNAs?piRNAs silence transposable elements in germ cellspiRNA is the abbreviation of $P$-element interacting RNApiRNAs modify ...

​​​​Which one of the following coenzymes is utilised by alanine racemase for the conversion of $L$-Alanine to $D$-Alanine?Pyridoxal phosphateThiamine pyrophosphateTetrahy...

​​​​Which of the following enzymes is/are involved in the biogenesis of miRNA?DroshaCas$9$XRCC$4$Dicer

​​​​​​Which of the following separation processes is/are based on molecular size?Size-exclusion chromatographyIon exchange chromatographyMembrane ultrafiltrationUltracent...

​​Which of the following show(s) optical activity at $100 \: \mathrm{mM}$ concentration in water?Solution of NaClSolution of D-GlucoseSolution of GlycineSolution of L-Pro...

​​​Which of the following compounds have the same degree of reduction per carbon-mole?GlucoseLactic acidAcetic acidFormic acid

The contour length of a B-DNA molecule that encodes a bacterial protein of $33 \: \text{kDa}$ is $\_\_\_\_$ $\mathrm{ nm}$.Consider the average molecular weight of an ami...

Within the Michaelis-Menten framework, the ratio of $v_{0} / V_{\max }$ when $[\mathrm{S}]=20 \times K_{\mathrm{m}}$ is $\_\_\_\_\_$(Round off to two decimal places)

Correctly match the Inhibitor with its respective Function in mitochondrial respiration.\[\begin{array}{|l|}\hline\begin{array}{l|l}\textbf{Inhibitor} & \textbf{Function}...

​​​​​An octapeptide composed of these $L$-amino acids - Lys, Thr, Ser, Met, Arg, Trp, Tyr, Glu - was subjected to analyses with the following outcomes:$\text{P}$. The N-t...

​​​Correctly match the type of Hypersensitivity reaction with its respective Example.$$\begin{array}{|l|l|}\hline \textbf{Hypersensitivity reaction} & \textbf{Example} \\...

Correctly match the Enzyme with its respective Function.\begin{array}{|l|l|}\hline \textbf{ Enzyme } & \textbf{ Function } \\\hline \text{P. Gyrase} & 1. \text{Removes a ...