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561
GATE2015-56
Assuming random distribution of nucleotides, the average number of fragments generated upon digestion of a circular DNA of size $4.3 \times 10^5$ bp with $\textit{Alu}I (5 ' – AG \downarrow CT-3' )$ is _________$ \times 10^3$
Assuming random distribution of nucleotides, the average number of fragments generated upon digestion of a circular DNA of size $4.3 \times 10^5$ bp with $\textit{Alu}I (...
Milicevic3306
7.9k
points
Milicevic3306
asked
Mar 26, 2018
Others
gate2015
numerical-answers
+
–
0
votes
0
answers
562
GATE2015-57
A synchronous culture containing $1.8 \times 10^5$ monkey kidney cells was seeded into three identical flasks. The doubling time of these cells is $24$ h. After $24$ h, the cells from all the three flasks were pooled and dispensed equally into each well of three $6$-well plates. The number of cells in each well will be _________$\times 10^4$.
A synchronous culture containing $1.8 \times 10^5$ monkey kidney cells was seeded into three identical flasks. The doubling time of these cells is $24$ h. After $24$ h, t...
Milicevic3306
7.9k
points
Milicevic3306
asked
Mar 26, 2018
Others
gate2015
numerical-answers
+
–
0
votes
0
answers
563
GATE2015-58
An $\textit{in vitro}$ translation system can synthesize peptides m all three reading frames of the RNA template. When $\text{5' - UCUCUCUC --- (UC)_n---UCUCUCUC - 3'}$ was used as the template in this $\textit{in vitro}$ translation system, the synthesized ... peptides contained $33.3 \%$ each of serine. leucine, and proline. Deduce the codon for proline. $UCU$ $CUC$ $CCU$ $UCC$
An $\textit{in vitro}$ translation system can synthesize peptides m all three reading frames of the RNA template. When $\text{5’ - UCUCUCUC - (UC)_n -UCUCUCUC - 3’}$...
Milicevic3306
7.9k
points
Milicevic3306
asked
Mar 26, 2018
Others
gate2015
+
–
0
votes
0
answers
564
GATE2015-59
Three distinct antigens $X, Y$ and $Z$ were used to raise antibodies. Antigen $Z$ was injected in a mouse on day zero followed by the administration of antigens $X$ and $Y$ on day $28$. A second injection of antigen $X$ was administered on day $70$. The antibody titers were ... will be high on day $84$ $X$-specific IgG will be high on day $42$ $Y$-specific IgG will be high on day $84$
Three distinct antigens $X, Y$ and $Z$ were used to raise antibodies. Antigen $Z$ was injected in a mouse on day zero followed by the administration of antigens $X$ and $...
Milicevic3306
7.9k
points
Milicevic3306
asked
Mar 26, 2018
Others
gate2015
+
–
0
votes
0
answers
565
GATE2015-60
The standard free energy change $(\Delta G' ^\circ)$ for ATP hydrolysis is $-30 kJ \cdot \text{mole}^{-1}$. The $\textit{in vivo}$ concentrations of ATP, ADP and $P_i$ in $E \textit{.coli}$ are $7.90, 1.04$ and $7.90$ ... $(\Delta G)$ for ATP hydrolysis $\textit{in viva}$ is _______ $kJ \cdot \text{mole}^{-1}$.
The standard free energy change $(\Delta G' ^\circ)$ for ATP hydrolysis is $-30 kJ \cdot \text{mole}^{-1}$. The $\textit{in vivo}$ concentrations of ATP, ADP and $P_i$ in...
Milicevic3306
7.9k
points
Milicevic3306
asked
Mar 26, 2018
Others
gate2015
numerical-answers
+
–
0
votes
0
answers
566
GATE2015-61
In a fed-batch culture, $200 \text{ g . L}^{-1}$ glucose solution is added at a flow rate of $50 \text{ L . h}^{-1}$ . The initial culture volume (at quasi steady state) and the initial cell concentration are $600 \: L$ and $20 \text{g . L}^{-1}$ , respectively. The ... g substrate$^{-1}$. The cell concentration $g . L^{-1})$ at quasi steady state at $t=8 \:h$ is $40$ $52$ $60$ $68$
In a fed-batch culture, $200 \text{ g . L}^{-1}$ glucose solution is added at a flow rate of $50 \text{ L . h}^{-1}$ . The initial culture volume (at quasi steady state) ...
Milicevic3306
7.9k
points
Milicevic3306
asked
Mar 26, 2018
Others
gate2015
+
–
0
votes
0
answers
567
GATE2015-62
Cytoplasmic extract from the wild type strain of a bacterium has the ability to convert a colorless substrate $(S)$ to a colored product $(P)$ via three colorless intermediates $X, Y$ and $Z$ in that order. Each step of the pathway involves a specific ... No: no color developed) Based on the data, which one of the following is the correct order of enzymes involved in the pathway?
Cytoplasmic extract from the wild type strain of a bacterium has the ability to convert a colorless substrate $(S)$ to a colored product $(P)$ via three colorless interme...
Milicevic3306
7.9k
points
Milicevic3306
asked
Mar 26, 2018
Others
gate2015
+
–
0
votes
0
answers
568
GATE2015-63
Samples of bacterial culture taken at $5$ PM and then the next day at $5$ AM were found to have $10^4$ and $10^7$ cells.mL${-1}$, respectively. Assuming that both the samples were taken during the log phase of cell growth, the generation time of this bacterium will be ________$h$.
Samples of bacterial culture taken at $5$ PM and then the next day at $5$ AM were found to have $10^4$ and $10^7$ cells.mL${-1}$, respectively. Assuming that both the sam...
Milicevic3306
7.9k
points
Milicevic3306
asked
Mar 26, 2018
Others
gate2015
numerical-answers
+
–
0
votes
0
answers
569
GATE2015-64
Biomass is being produced in a continuous stirred tank bioreactor of $750$ L capacity. The sterile feed containing $8 \: g \cdot L^{-1}$ glucose as substrate was fed at a flow rate of $150 \: L \cdot h^{-1}$ ... Determine the cell productivity $(g \cdot L^{-1} \cdot h^{-1})$ at steady state. $0.85$ $0.65$ $0.45$ $0.25$
Biomass is being produced in a continuous stirred tank bioreactor of $750$ L capacity. The sterile feed containing $8 \: g \cdot L^{-1}$ glucose as substrate was fed at a...
Milicevic3306
7.9k
points
Milicevic3306
asked
Mar 26, 2018
Others
gate2015
+
–
0
votes
0
answers
570
GATE2015-65
A linear double stranded DNA of length $8$ kbp has three restriction sites. Each of these can either be a $\textit{Bam}\text{HI}$ or a $\textit{Hae}III$ site. The DNA was digested completely with both enzymes. The products were ... autoradiograph represent their mean signal intensity in arbitrary units. Which one of the following options is the correct restriction map of the DNA?
A linear double stranded DNA of length $8$ kbp has three restriction sites. Each of these can either be a $\textit{Bam}\text{HI}$ or a $\textit{Hae}III$ site. The DNA was...
Milicevic3306
7.9k
points
Milicevic3306
asked
Mar 26, 2018
Others
gate2015
+
–
0
votes
0
answers
571
GATE2014-GA-1
Choose the most appropriate word from the options given below to complete the following sentence. A person suffering from Alzheimer’s disease___________ short-term memory loss. experienced has experienced is experiencing experiences
Choose the most appropriate word from the options given below to complete the following sentence.A person suffering from Alzheimer’s disease___________ short-term memor...
Milicevic3306
7.9k
points
Milicevic3306
asked
Mar 25, 2018
Verbal Aptitude
gate2014
verbal-ability
most-appropriate-word
+
–
0
votes
0
answers
572
GATE2014-GA-2
Choose the most appropriate word from the options given below to complete the following sentence. ___________is the key to their happiness; they are satisfied with what they have. Contentment Ambition Perseverance Hunger
Choose the most appropriate word from the options given below to complete the following sentence.___________is the key to their happiness; they are satisfied with what th...
Milicevic3306
7.9k
points
Milicevic3306
asked
Mar 25, 2018
Verbal Aptitude
gate2014
verbal-ability
most-appropriate-word
+
–
0
votes
0
answers
573
GATE2014-GA-3
Which of the following options is the closest in meaning to the sentence below? “As a woman, I have no country.” Women have no country. Women are not citizens of any country. Women’s solidarity knows no national boundaries. Women of all countries have equal legal rights.
Which of the following options is the closest in meaning to the sentence below?“As a woman, I have no country.”Women have no country.Women are not citizens of any cou...
Milicevic3306
7.9k
points
Milicevic3306
asked
Mar 25, 2018
Verbal Aptitude
gate2014
verbal-ability
verbal-reasoning
normal
+
–
0
votes
0
answers
574
GATE2014-GA-4
In any given year, the probability of an earthquake greater than Magnitude $6$ occurring in the Garhwal Himalayas is $0.04$. The average time between successive occurrences of such earthquakes is_____ years.
In any given year, the probability of an earthquake greater than Magnitude $6$ occurring in the Garhwal Himalayas is $0.04$. The average time between successive occurrenc...
Milicevic3306
7.9k
points
Milicevic3306
asked
Mar 25, 2018
Quantitative Aptitude
gate2014
numerical-ability
probability
numerical-answers
normal
+
–
0
votes
0
answers
575
GATE2014-GA-5
The population of a new city is $5$ million and is growing at $20\%$ annually. How many years would it take to double at this growth rate? $3-4$ years $4-5$ years $5-6$ years $6-7$ years
The population of a new city is $5$ million and is growing at $20\%$ annually. How many years would it take to double at this growth rate?$3-4$ years$4-5$ years$5-6$ year...
Milicevic3306
7.9k
points
Milicevic3306
asked
Mar 25, 2018
Quantitative Aptitude
gate2014
numerical-ability
simple-compound-interest
normal
+
–
0
votes
0
answers
576
GATE2014-GA-6
In a group of four children, Som is younger to Riaz. Shiv is elder to Ansu. Ansu is youngest in the group. Which of the following statements is/are required to find the eldest child in the group? Statements 1. Shiv is younger to Riaz. 2. ... child Statements 1 and 2 are both required to determine the eldest child Statements 1 and 2 are not sufficient to determine the eldest child
In a group of four children, Som is younger to Riaz. Shiv is elder to Ansu. Ansu is youngest in the group. Which of the following statements is/are required to find the e...
Milicevic3306
7.9k
points
Milicevic3306
asked
Mar 25, 2018
Analytical Aptitude
gate2014
analytical-aptitude
logical-reasoning
statements-follow
+
–
0
votes
0
answers
577
GATE2014-GA-7
Moving into a world of big data will require us to change our thinking about the merits of exactitude. To apply the conventional mindset of measurement to the digital, connected world of the twenty-first century is to miss a crucial point. As mentioned ... data is obsessed with exactness Exactitude is not critical in dealing with big data Sparse data leads to a bias in the analysis
Moving into a world of big data will require us to change our thinking about the merits of exactitude. To apply the conventional mindset of measurement to the digital, co...
Milicevic3306
7.9k
points
Milicevic3306
asked
Mar 25, 2018
Verbal Aptitude
gate2014
verbal-ability
verbal-reasoning
passage-reading
+
–
0
votes
0
answers
578
GATE2014-GA-8
Q8The total exports and revenues from the exports of a country are given in the two pie charts below.The pie chart for exports shows the quantity of each item as a percentage of the total quantity ofexports. The pie chart for the revenues shows the percentage of ... of Item 1per kilogram to the revenue generated through export of Item 4 per kilogram?Exports Revenues 1:2 2:1 1:4 4:1
Q8The total exports and revenues from the exports of a country are given in the two pie charts below.The pie chart for exports shows the quantity of each item as a percen...
Milicevic3306
7.9k
points
Milicevic3306
asked
Mar 25, 2018
Quantitative Aptitude
gate2014
numerical-ability
data-interpretation
pie-chart
+
–
0
votes
0
answers
579
GATE2014-GA-9
X is $1$ km northeast of Y. Y is $1$ km southeast of Z. W is $1$ km west of Z. P is $1$ km south of W. Q is $1$ km east of P. What is the distance between X and Q in km? $1$ $\sqrt{2}$ $\sqrt{3}$ $2$
X is $1$ km northeast of Y. Y is $1$ km southeast of Z. W is $1$ km west of Z. P is $1$ km south of W. Q is $1$ km east of P. What is the distance between X and Q in km?$...
Milicevic3306
7.9k
points
Milicevic3306
asked
Mar 25, 2018
Analytical Aptitude
gate2014
analytical-aptitude
direction-sense
+
–
0
votes
0
answers
580
GATE2014-GA-10
$10\%$ of the population in a town is HIV+. A new diagnostic kit for HIV detection is available; this kit correctly identifies HIV+ individuals 95% of the time, and HIV individuals $89\%$ of the time. A particular patient is tested using this kit and is found to be positive. The probability that the individual is actually positive is_______
$10\%$ of the population in a town is HIV+. A new diagnostic kit for HIV detection is available; this kit correctly identifies HIV+ individuals 95% of the time, and HIV i...
Milicevic3306
7.9k
points
Milicevic3306
asked
Mar 25, 2018
Quantitative Aptitude
gate2014
numerical-ability
probability
conditional-probability
numerical-answers
+
–
0
votes
0
answers
581
GATE2014-1
The eigenvalues of A =$\begin{bmatrix} 1&-4\\2 & -3 \end{bmatrix} $are $2\underline{+}i$ $-1, -2$ $-1\underline{+}2i$ non-existent
The eigenvalues of A =$\begin{bmatrix} 1&-4\\2 & -3 \end{bmatrix} $are$2\underline{+}i$$-1, -2$$-1\underline{+}2i$non-existent
Milicevic3306
7.9k
points
Milicevic3306
asked
Mar 25, 2018
Linear Algebra
gate2014
linear-algebra
matrices
eigen-values
+
–
0
votes
0
answers
582
GATE2014-2
If an unbiased coin is tossed $10$ times, the probability that all outcomes are same will be_______ $ \times 10^{-5}$
If an unbiased coin is tossed $10$ times, the probability that all outcomes are same will be_______ $ \times 10^{-5}$
Milicevic3306
7.9k
points
Milicevic3306
asked
Mar 25, 2018
Probability and Statistics
gate2014
probability-and-statistics
probability
conditional-probability
numerical-answers
+
–
0
votes
0
answers
583
GATE2014-3
The solution for the following set of equations is, $5x+4y+102=13$ $x+3y+z=7$ $4x2y+z=0$ $x=2,y=1,z=1$ $x=1,y=2,z=0$ $x=1,y=0,z=2$ $x=0,y=1,z=2$
The solution for the following set of equations is,$$5x+4y+102=13$$$$x+3y+z=7$$$$4x2y+z=0$$$x=2,y=1,z=1$$x=1,y=2,z=0$$x=1,y=0,z=2$$x=0,y=1,z=2$
Milicevic3306
7.9k
points
Milicevic3306
asked
Mar 25, 2018
Linear Algebra
gate2014
linear-algebra
system-of-equations
+
–
0
votes
0
answers
584
GATE2014-4
The limit of the function $e^{-2t}\sin (t)$ as t $\rightarrow\infty$ so, is
The limit of the function $e^{-2t}\sin (t)$ as t $\rightarrow\infty$ so, is
Milicevic3306
7.9k
points
Milicevic3306
asked
Mar 25, 2018
Calculus
gate2014
calculus
limits
numerical-answers
+
–
0
votes
0
answers
585
GATE2014-5
The solution to the following set of equations is $2x+3y=4$ $4x+6y=0$ $x=0,y=0$ $x=2, y=0$ $4x=-6y$ No solution
The solution to the following set of equations is$$2x+3y=4$$$$4x+6y=0$$$x=0,y=0$$x=2, y=0$$4x=-6y$No solution
Milicevic3306
7.9k
points
Milicevic3306
asked
Mar 25, 2018
Linear Algebra
gate2014
linear-algebra
system-of-equations
+
–
0
votes
0
answers
586
GATE2014-6
The unit for specific substrate consumption rate in a growing culture is $\frac{g}{L-h}$ $\frac{g}{h}$ $\frac{g}{g-h}$ $\frac{gmoles}{L-h}$
The unit for specific substrate consumption rate in a growing culture is$\frac{g}{L-h}$$\frac{g}{h}$$\frac{g}{g-h}$$\frac{gmoles}{L-h}$
Milicevic3306
7.9k
points
Milicevic3306
asked
Mar 25, 2018
Others
gate2014
numerical-estimation
+
–
0
votes
0
answers
587
GATE2014-7
If the dissociation constant for solute-adsorbent binding is $K_D$, the retention time of the solute in a chromatography column increases with increasing $K_D$ decreases with increasing $K_D$ passes through minimum with increasing $K_D$ is independent of $K_D$
If the dissociation constant for solute-adsorbent binding is $K_D$, the retention time of the solute in a chromatography columnincreases with increasing $K_D$decreases wi...
Milicevic3306
7.9k
points
Milicevic3306
asked
Mar 25, 2018
Biochemistry
gate2014
+
–
0
votes
0
answers
588
GATE2014-9
The most plausible explanation for a sudden increase of the respiratory quotient $(RQ)$ of a microbial culture is that cells are dying yield of biomass is increasing the fermentation rate is increasing relative to respiration rate the maintenance rate is decreasing
The most plausible explanation for a sudden increase of the respiratory quotient $(RQ)$ of a microbial culture is thatcells are dyingyield of biomass is increasingthe fer...
Milicevic3306
7.9k
points
Milicevic3306
asked
Mar 25, 2018
Microbiology
gate2014
microbial-growth
+
–
0
votes
0
answers
589
GATE2014-10
Which of the following is employed for the repeated use of enzymes in bioprocesses? polymerization immobilization ligation isomerization
Which of the following is employed for the repeated use of enzymes in bioprocesses?polymerizationimmobilizationligationisomerization
Milicevic3306
7.9k
points
Milicevic3306
asked
Mar 25, 2018
Bioprocess Engineering and Process Biotechnology
gate2014
enzymes
+
–
0
votes
0
answers
590
GATE2014-11
Since mammalian cells are sensitive to shear, scale-up of a mammalian cell process must consider, among other parameters, the following $\text{(given N=rotations/time, D=diameter of impeller)}$ $\pi ND$ $\pi N^2D$ $\pi ND^2$ none of these
Since mammalian cells are sensitive to shear, scale-up of a mammalian cell process must consider, among other parameters, the following $\text{(given N=rotations/time, D=...
Milicevic3306
7.9k
points
Milicevic3306
asked
Mar 25, 2018
Others
gate2014
+
–
0
votes
0
answers
591
GATE2014-12
The degree of reduction of ethanol is_______
The degree of reduction of ethanol is_______
Milicevic3306
7.9k
points
Milicevic3306
asked
Mar 25, 2018
Bioprocess Engineering and Process Biotechnology
gate2014
degree-of-reduction-of-elements
numerical-answers
+
–
0
votes
0
answers
592
GATE2014-13
Gram-positive bacteria are generally resistant to complement-mediated lysis because thick peptidoglycan layer prevents insertion of membrane attack complex into the irmer membrane Gram-positive bacteria import the membrane attack complex and inactivate it ... degraded by the proteases produced by the Gram-positive bacteria Gram-positive bacteria cannot activate the complement pathway
Gram-positive bacteria are generally resistant to complement-mediated lysis becausethick peptidoglycan layer prevents insertion of membrane attack complex into the irmer ...
Milicevic3306
7.9k
points
Milicevic3306
asked
Mar 25, 2018
Microbiology
gate2014
bacterial
+
–
0
votes
0
answers
593
GATE2014-14
A bacterium belonging to cocci group has a diameter of $2\:\mu m.$ The numerical value of the ratio of its surface area to volume (in $\mu m^{-1}$) is _______.
A bacterium belonging to cocci group has a diameter of $2\:\mu m.$ The numerical value of the ratio of its surface area to volume (in $\mu m^{-1}$) is _______.
Milicevic3306
7.9k
points
Milicevic3306
asked
Mar 25, 2018
Others
gate2014
numerical-answers
+
–
0
votes
0
answers
594
GATE2014-15
Which of the following essential element(s) is/are required as major supplement to enhance the bioremediation of oil spills by the resident bacteria? Sulfur Nitrogen and phosphorus Iron Carbon
Which of the following essential element(s) is/are required as major supplement to enhance the bioremediation of oil spills by the resident bacteria?SulfurNitrogen and ph...
Milicevic3306
7.9k
points
Milicevic3306
asked
Mar 25, 2018
Microbiology
gate2014
bacterial
+
–
0
votes
0
answers
595
GATE2014-16
The 4-amino or 4-keto group of pyrimidine bases is located in the major groove of the double stranded DNA minor groove of the double stranded DNA minor groove of the B form DNA but not the A form DNA major groove of the B form DNA but not the A form DNA
The 4-amino or 4-keto group of pyrimidine bases is located in themajor groove of the double stranded DNAminor groove of the double stranded DNAminor groove of the B form ...
Milicevic3306
7.9k
points
Milicevic3306
asked
Mar 25, 2018
Recombinant DNA technology and Other Tools in Biotechnology
gate2014
dna
+
–
0
votes
0
answers
596
GATE2014-17
The product(s) resulting from the hydrolysis of maltose is/are a mixture of $\alpha$-D-Glucose and $\beta$-D-Glucose a mixture of D-Glucose and L-Glucose $\alpha$-D-Glucose only $\beta$-D-Glucose only
The product(s) resulting from the hydrolysis of maltose is/area mixture of $\alpha$-D-Glucose and $\beta$-D-Glucosea mixture of D-Glucose and L-Glucose$\alpha$-D-Glucose ...
Milicevic3306
7.9k
points
Milicevic3306
asked
Mar 25, 2018
Biochemistry
gate2014
glucose
+
–
0
votes
0
answers
597
GATE2014-18
Amino acid residue which is most likely to be found in the interior of water-soluble globular proteins is Threonine Aspartic acid Valine Histidine
Amino acid residue which is most likely to be found in the interior of water-soluble globular proteins isThreonineAspartic acidValineHistidine
Milicevic3306
7.9k
points
Milicevic3306
asked
Mar 25, 2018
Biochemistry
gate2014
aminoacids
+
–
0
votes
0
answers
598
GATE2014-19
The $5$’ ends of the mature forms of the prokaryotic mRNAs and tRNAs are a triphosphate group in mRNAs and a monophosphate group in tRNAs triphosphate groups in both mRNAs and tRNAs monophosphate groups in both mRNAs and tRNAs a monophosphate group in mRNAs and a triphosphate group in tRNAs
The $5$’ ends of the mature forms of the prokaryotic mRNAs and tRNAs area triphosphate group in mRNAs and a monophosphate group in tRNAstriphosphate groups in both mRNA...
Milicevic3306
7.9k
points
Milicevic3306
asked
Mar 25, 2018
Recombinant DNA technology and Other Tools in Biotechnology
gate2014
cdna-and-dna
+
–
0
votes
0
answers
599
GATE2014-20
Prior exposure of plants to pathogens is known to increase resistance to future pathogen attacks.This phenomenon is known as systemic acquired resistance hypersensitive response innate immunity antibody mediated response
Prior exposure of plants to pathogens is known to increase resistance to future pathogen attacks.This phenomenon is known assystemic acquired resistancehypersensitive res...
Milicevic3306
7.9k
points
Milicevic3306
asked
Mar 25, 2018
Immunology
gate2014
history-of-immunology
+
–
0
votes
0
answers
600
GATE2014-21
Reactions between antibodies and antigens that are detected by precipitate formation in an agar gel are referred as immunoprecipitation assay immunodiffusion assay immunoaggregation assay immunofixation assay
Reactions between antibodies and antigens that are detected by precipitate formation in an agar gel are referred asimmunoprecipitation assayimmunodiffusion assayimmunoagg...
Milicevic3306
7.9k
points
Milicevic3306
asked
Mar 25, 2018
Immunology
gate2014
antigen-antibody-reactions
+
–
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