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GATE2016-40

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In animal cell culture, a CO$_2$ enriched atmosphere in the incubator chamber is used to maintain the culture pH between $6.9$ and $7.4$. Which one of the following statements is CORRECT?

  1. Higher the bicarbonate concentration in the medium, higher should be the requirement of gaseous CO$_2$
  2. Lower the bicarbonate concentration in the medium, higher should be the requirement of gaseous CO$_2$
  3. Higher the bicarbonate concentration in the medium, lower should be the requirement of gaseous CO$_2$
  4. CO$_2$ requirement is independent of bicarbonate concentration in the medium
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