Questions without answers in Recombinant DNA Technology

Which component of the $\text{CRISPR/Cas9}$ gene editing system is $\text{NOT}$ of natural origin?$\text{CAS9}$ protein$\text{CRISPR}$ repeats$\text{PAM}$ sequence$\text{...

​​​​​Which one of the following hosts is used in mammalian cell culture for the production of glycosylated recombinant therapeutic proteins?Pichia pastorisSf$9$ cellsEsch...

Which of the following vector(s) is(are) used to clone a DNA fragment of size $220 \mathrm{~kb}$?Bacterial artificial chromosomeYeast artificial chromosomeCosmids$\text{p...

Introduction of foreign genes into plant cells can be carried out usingAgrobacterium$\text{Cacl}_{2}$ mediated plasmid uptakeElectroporationGene gun

The schematic of a plasmid with a gap in one of the strands is shown below:Which of the following enzyme(s) is/are required to fill the gap and generate a covalently clos...

Determine the correctness or otherwise of the following Assertion [$a$] and the Reason [$r$].Assertion [$a$]: A genetically engineered rice that produces beta-carotene in...

During a positive-negative selection process, transformed animal cells expressing __________________ are killed in presence of ganciclovir in the medium.Pyruvate kinasevi...

Select the CORRECT combination of genetic components that are essential for the transfer of T-DNA segment from Agrobacterium tumefaciens to plant cells.Border repeat sequ...

A $1.2$ kb DNA fragment was cloned into $\textit{Bam}$HI and $\textit{Eco}$RI sites located on a $2.8$ kb cloning vector. The $\textit{Bam}$HI and $\textit{Eco}$RI sites ...

A recombinant protein is to be expressed under the control of the lac promoter and operator in a strain $\textit{E.coli}$ having the genotype lacI$^+$ crp$^+$ . Even in t...

Shown below id a plasmid vector (P) and an insert (Q). The insert was cloned into the BamHI site of the vector. The recombinant plasmid was isolated and digested with Bam...

Which one of the following is a second generation genetically engineered crop?Bt brinjalRoundup soyabeanGolden riceBt rice

Which one of the following features is NOT required in a prokaryotic expression vector?$\textit{oriC}$Selection markerCMV promoterRibosome binding site

The basis for blue-white screening with $pUC$ vectors isintraallelic complementationintergenic complementationintragenic suppressionextragenic suppression