Recent questions in General Biology

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Answer the question based on the information given below:The purification data for an enzyme is given below:$\begin{array}{|l|l|l|l|l|l|} \hline & \text{Step} & \text{Vol...
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Answer the question based on the information given below:The purification data for an enzyme is given below:$\begin{array}{|l|l|l|l|l|l|} \hline & \text{Step} & \text{Vol...
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$\text{Statement for Linked Answer Questions 52 and 53:}$An E $coli$ cell of volume $10^{-12}$ $cm^3$ contains 60 molecules of lac-repressor. The repressor has a binding ...
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$\text{Statement for Linked Answer Questions 52 and 53:}$An E $coli$ cell of volume $10^{-12}$ $cm^3$ contains 60 molecules of lac-repressor. The repressor has a binding ...
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Match the high energy compounds in $\text{Group I}$ with the biosynhetic pathways for the molecules in $\text{Group II}$.$\begin{array}{|l|l|l|l|} \hline & \text{Group I...
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Match the vitamins in $\text{Group I}$ with the processes/reactions in $\text{Group II}$$\begin{array}{|l|l|l|l|} \hline & \text{Group I} && \text{Group II} \\ \hline P. ...
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An enzymatic reaction is described by the following rate expression.$$v = \frac{v_mS}{K_m+S+S^2/K_s},$$Which one of the following curves represents this expression?
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A bacterial culture ($200 \: \mu l$ containing $1.8 \times 10^9$ cells) was treated with an antibiotic $Z$ ($50 \: \mu g$ per $ml$) for $4$ h at $37^\circ C$. After this ...
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In a muscle, the extracellular and intracellular concentrations of ${Na^+}$ are $150 \text{ mM}$ and $12 \text{ mM}$, and those of ${K^+}$ are $2.7 \text{ mM}$ and $140 \...
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$\text{Common Data Questions}$$\text{Common Data for Questions 48 and 49}$:In a muscle, the extracellular and intracellular concentrations of ${Na^+}$ are $\text{150 mM}$...
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Match the organisms in Group I with the entries in Group II.$\begin{array}{|l|l|l|l|} \hline & \textbf{Group I}. && \textbf{Group II} \\ \hline P. & \textit{Clostridium...
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Match the entries in $\text{Group I}$ with the methods of sterilization in $\text{Group II}$$\begin{array}{|l|l|l|l|} \hline & \textbf{Group I} && \text{Group II} \\ \h...
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Match the entries in $\textbf{Group I}$ with those in $\textbf{Group II}$.$\begin{array}{|c|l|c|l|}\hline &\textbf{Group I} & {} & \textbf{Group II} \\\hline P. & \text{M...
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A single base pair of DNA weighs $1.1 \times 10^{-21}$ grams. How many picomoles of a plasmid vector of length $2750$ bp are contained in 1 $\mu$g of purified DNA?$0.30$$...
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Synteny refers togene duplication from a common ancestora tree representation of related sequencesthe extent of similarity between two sequenceslocal conservation of gene...
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While searching a database for similar sequences, E value does NOT depend on thesequence lengthnumber of sequences in the databasescoring systemprobability from a normal ...
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In transmission electron microscopy, electron opacity is greatly enhanced by treating the specimen withferrous ammonium sulfateuranium acetatesodium chloridebasic fuchsin
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The helix content of a protein can be determined usingan infrared spectrometera fluorescence spectrometera circular dichroism spectrometera UV-Visible spectrophotometer
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Which one of the following DNA sequences carries an invert repeat?ATGAGCCCCGAGTATACTCGGGGCTCATATGAGCCGGCTCTATACTCGGCCGAGATATGAGCCGAGCCTAACTCGGCTCGGATATGAGCCTATGGTATACTCGG...
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In zinc finger proteins, the amino acid residues that coordinate zinc areCys and HisAsp and GluArg and LysAsp and Arg
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The activity of an enzyme is expressed in International Units (IU). However, the S.I unit for enzyme activity is Katal. One Katal is$1.66 \times {10^4}$ IU$60$ IU$6 \time...
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Identify the statement that is NOT applicable to an enzyme catalyzed reaction.Enzyme catalysis involves propinquity effectsThe binding of substrate to the active site cau...
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An example of a derived protein structure database isPfamSCOPGEOProsite
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An example of a program for constructing a phylogenetic tree isphylipphrapprodomPHDsec
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A humanized antibody is one in which theheavy and light chains are from humanheavy chain is from human and light chain is from mouselight chain is from human and heavy ch...
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Dimethyl sulfoxide (DMSO) is used as a cryopreservant for mammalian cell cultures becauseit is an organic solventit easily penetrates cellsit protects cells by preventing...
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Nude mice refers tomice without skinmice without thymusknockout micetransgenic mice
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Heat inactivation of serum is done to inactivateprionsmycoplasmacomplementpathogenic bacteria
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Choose the correct signal transduction pathway.Hormone $\to$ 7 TM receptor $\to$ G protein $\to$ cAMP $\to$ PKAHormone $\to$ G protein $\to$ 7 TM receptor $\to$ cAMP $\t...
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A protein is phosphorylated at a serine residue. A phosphomimic mutant of the protein can be generated by substituting that serine withglycinealanineaspartatethreonine
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A truncated polypeptide is synthesized due to a nonsense mutation. Where would you introduce another mutation to obtain a full-length polypeptide?Ribosomal protein geneTr...
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Idiotypic determinants of an antibody are associated with theconstant region of the heavy chainsconstant region of the light chainsvariable regionconstant regions of ligh...
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Identification of blood groups involvesprecipitationneutralizationopsonizationagglutination
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B-lymphocytes originate from the bone marrow whereas T-lymphocytes originate fromthymusbone marrowspleenliver
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In mismatch correction repair, the parental DNA strand is distinguished from the daughter strand byacetylationphosphorylationmethylationglycosylation
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In a chemostat, the feed flow rate and culture volume are $100$ ml/h and $1.0$ L, respectively. With glucose as substrate, the values of $\mu_{\text{max}}$ and $K_s$ are ...
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Mammalian cells in active growth phase were seeded at a density of $1 \times 10^5$ cells/ml. After $72$ hours, $1 \times 10^6$ cells/ml were obtained. The population doub...
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Yeast converts glucose to ethanol and carbon dioxide by glycolysis as per the following reaction: $$C_6H_{12}O_6 \to 2C_2H_5OH + 2CO_2$$Assuming complete conversion, the ...
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Consider the following alignment of two DNA sequences:$$\mathbf{AGTAAC} \\ \mathbf{AA}\text{- -}\mathbf{AC}$$Assuming an affine gap scoring scheme of an identity matrix f...
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First order deactivation rate constants for soluble and immobilized amyloglucosidase enzyme are $0.03 \text{ min}^{-1}$ and $0.005 \text{ min}^{-1}$, respectively. The ra...