Recent questions in Recombinant DNA technology and Other Tools in Biotechnology

The $5$’ ends of the mature forms of the prokaryotic mRNAs and tRNAs area triphosphate group in mRNAs and a monophosphate group in tRNAstriphosphate groups in both mRNAs ...

The plasmid DNA was subjected to restriction digestion using the enzyme $\textit{EcoRI}$ and analysed on an agarose gel. Assuming digestion has worked (the enzyme was act...

A DNA fragment of 5000bp needs to be isolated from $E.coli$ (genome size $4\times10^3 kb$) genomic library.The minimum number of independent recombinant clones required t...

A complete restriction digestion of a circular plasmid $(5000bp)$ was carried out with $HindIII,BamHIandEcoRIindividually.$ Restriction digestion yielded following fragme...

The suitable substitution matrix to align closely related sequences is$\text{PAM 250 or BLOSSUM 80}$$\text{PAM 40 or BLOSSUM 80}$$\text{PAM 120 or BLOSSUM 40}$$\text{PAM ...

The $RNA$ primer synthesized during the replication process in bacteria is removed by$DNA$ gyraseprimase$DNA$ polymerase $I$$DNA$ polymerase $II$

Of the two diploid species, species $I$ has $36$ chromosomes and species $II$ has $28$ chromosomes. How many chromosomes would be found in an allotriploid individual?$42$...

Phylum proteobacteria is subdivided into $\alpha-, \beta-, \gamma-, \delta-$ and $\epsilon-$ proteobacteria based on$G+C$ content$23S\; rRNA$ sequences$tRNA$ sequences$16...

In nature, the horizontal gene transfer across bacteria is mediated bygene cloning followed by transformationconjugation and transformationconjugation onlytransformation ...

The nucleotide analogue used in DNA sequencing by chain termination method is$l',3'-$dideoxy nucleoside triphosphate$2',3'-$dideoxy nucleoside triphosphate$2',4'-$dideoxy...

Human genome sequencing project involved the construction of genomic library inbacterial artificial chromosome$pBR322$bacteriophage$pcDNA3.1$

Which one of the following is NOT a protoplast fusion inducing agent?Inactivated Sendai virus$Ca^{2+}$ at alkaline pHPolyethylene glycolColchicine

Protein-DNA interactions $\text{in vivo}$ can be studied bygel shift assaySouthern hybridizationchromatin immunoprecipitation assayfluorescence $\text{in situ}$ hybridiza...

The basis for blue-white screening with $pUC$ vectors isintraallelic complementationintergenic complementationintragenic suppressionextragenic suppression

The product of complete digestion of the plasmid shown below with EcoRI and HaeIII was purified and used as a template in a reaction containing Klenow fragment of DNA pol...

Match the classes of RNA molecules in $\text{Group I}$ with their functions in $\text{Group II}$.$\begin{array}{|l|l|l|l|} \hline & \text{Group I} && \text{Group II} \\ ...

Genomic DNA isolated from a bacterium was digested with a restriction enzyme that recognizes a $6$-base pair (bp) sequence. Assuming random distribution of bases, the ave...

Which one of the following techniques is used to monitor RNA transcripts, both temporally and spatially?Northern blottingIn situ hybridizationSouthern blottingWestern blo...