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Match the item (Column I) with its corresponding use (Column II).

\begin{array}{|c|c|c|}
\hline \text{Column I} & \text{Column II} \\
\hline \text{P. Glutamine} & 1. \text{Detachment of adherent cells} \\
\hline \text{Q. Trypsin} & 2. \text{Selection of transfected mammalian cell lines} \\
\hline \text{R. Hypoxanthine} & 3. \text{Source of carbon and nitrogen in animal cell culture media}\\
\hline \text{S. Neomycin} & 4. \text{A component of medium for selection of hybridoma in monoclonal antibody production}\\
\hline
\end{array}

  1. $\text{P-3; Q-1; R-4; S-2}$
  2. $\text{P-1; Q-2; R-4; S-3}$
  3. $\text{P-3; Q-1; R-2; S-4}$
  4. $\text{P-2; Q-3; R-1; S-4}$
  5. $\text{P-3; Q-1; R-4; S-2}$

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