Recent activity in Analytical Techniques

The electron equivalent per liter (e-eq $\mathrm{L}^{-1}$ ) of acetate ion solution of concentration $10 \mathrm{~g} \mathrm{~L}^{-1}$ is $\_\_\_\_$ $\mathrm{e}^{-} \math...

You have purified an enzyme using a series of chromatographic methods. It was observed that a $10 ~\mu \mathrm{g} \mathrm{~mL}^{-1}$ of this purified enzyme converted $10...

Which one of the following proteins would elute third in gel filtration chromatography from a mixture of four proteins given below?Insulin $(6 \text{ kDa})$Myoglobin $(17...

Cancer cells display a high level of heterogeneity in the expression of TP$53$ protein. Which of the following techniques can be directly used to determine the intratumor...

An NMR spectrometer operating at proton resonance frequency $(v)$ of $1 \: \mathrm{GHz}$ will have a magnetic field strength of $\_\_\_\_$ Tesla (T).The gyromagnetic rati...

The percentage of light that would pass through a sample with an absorbance of $2$ would be $\_\_\_\_\_$ $\%$ .(Round off to the nearest integer)

Within the Michaelis-Menten framework, the ratio of $v_{0} / V_{\max }$ when $[\mathrm{S}]=20 \times K_{\mathrm{m}}$ is $\_\_\_\_\_$(Round off to two decimal places)

​​​​​​Which of the following separation processes is/are based on molecular size?Size-exclusion chromatographyIon exchange chromatographyMembrane ultrafiltrationUltracent...

For the coupled reactions given below\[\begin{array}{ll}\text { Glucose 6-phosphate }+\mathrm{H}_{2} \mathrm{O} \rightarrow \text { Glucose }+\mathrm{Pi} & \text { (React...

In adsorption chromatography, the adsorption of uncharged solute molecules onto a silica-based stationary phase is by $\_\_\_\_\_\_\_$.covalent bondselectrostatic interac...

Match the item in Column I with the corresponding technique in Column II.\begin{array}{|c|c|c|}\hline & \text{Column I} & \text{Column II} \\\hline & \text{P. Blue laser}...

Which of the following statements is/are correct about an uncompetitive inhibitor of an enzyme?It binds to the substrate binding site of the enzyme onlyIt binds to the en...

In ELISA, which of the following enzymes are conjugated to antibodies for detection of the analyte?P. Alkaline phosphataseQ. TrypsinaseR. Horseradish peroxidaseS. Amylase...

The distance between the two points of intersection of $x^{2}+y=7$ and $x+y=7$ (rounded off to two decimal places) is ___________.

Temperature of a reaction with an activation energy value of $15 \mathrm{kcal} \mathrm{mol}^{-1}$ is increased from $300 \mathrm{~K}$ to $310 \mathrm{~K}$. If the value o...

E. coli cultivated at $298 \mathrm{~K}$ uptakes an uncharged compound (A) by passive diffusion. The intracellular and extracellular concentrations of $\mathrm{A}$ are $0...

If $1000 \mathrm{bp}$ of a double-helical DNA weighs $1 \times 10^{-18} \mathrm{gm}$ and distance between two $\mathrm{bp}$ is $0.34 \mathrm{~nm}$, the total amount of DN...

A protein has three identical sites arranged at the vertices of an equilateral triangle. If one site is filled with a dye (donor), the measured quantum yield $\left(\phi_...

A dNTP master-mix is prepared by combining $40 \mu L$ of each $20 \mathrm{mM}$ dNTP stock (dATP, dCTP, dGTP and dTTP). $4 \mu L$ of this dNTP master-mix is added to a PCR...

Match the stationary phase (Column I) with its corresponding chromatography technique (Column II).$$\begin{array} {lll} \textbf{Column I} & & \textbf{Column II} \\ \text{...

Which of the following spectroscopic technique(s) can be used to identify all the functional groups of an antibiotic contaminant in food?InfraredCircular dichroismNuclear...

Which of the following nucleus/nuclei is/are $\text{NMR}$ active?$^1H$$^{13}C$$^{16}O$$^{32}S$

A $\text{DNA}$ solution of $50 \: \mu g \: mL^{-1}$ concentration gives an absorbance of $1.0$ at $260 \textit{ nm}$. An aliquot of $20 \: \mu L$ from a $50 \: \mu L$ pur...

The number of molecules of a nucleotide of molecular weight $300\:g$/mol present in $10$ picomoles is ____________ $\times10^{12}$ (round off to $2$ decimal places).

The concentrations of $ATP$, $ADP$ and inorganic phosphate in a cell are $2.59, \:0.73$ and $2.72 \:mM$, respectively. Under these conditions, free energy change for the ...

Two monomeric His-tagged proteins of identical molecular weight are present in a solution. pIs of these two proteins are $5.6$ and $6.8$. Which one of the following techn...

Solvents $A$ and $B$ are completely immiscible. Solute $S$ is soluble in both these solvents. $100\: g$ of $S$ was added to a container which has $2\: kg$ each of $A$ and...

Protein concentration of a crude enzyme preparation was $10\:mg\:mL^{-1}$. $10\:\mu L$ of this sample gave an activity of $5\:\mu mol\:min^{-1}$ under standard assay cond...

The hexapeptide $P$ has an isoelectric point $(pI)$ of $6.9$. Hexapeptide $Q$ is a variant of $P$ that contains valine instead of glutamate at position $3$. The two pepti...

$Group\; I$ lists spectroscopic methods and $Group\; II$ lists biomolecular applications of these methods. Match the methods in $Group\; I$ with the applications in $Grou...

The difference in concentrations of an uncharged solute between two compartments is $1.6$-fold. The energy required for active transport of the solute across the membrane...

A UV-visible spectrophotometer has a minimum detectable absorbance of $0.02$. The minimum concentration of a protein sample that can be measured reliably in this instrume...

In a thin layer chromatography experiment using a silica gel plate, a compound showed migration of $12.5$ cm and the solvent front showed migration of $18$ cm. The $R_f$ ...

Which one of the following techniques can be used to determine the structure of a $15$ kDa globular protein at atomic resolution?Raman spectroscopyIR spectroscopyUV spect...

Which one of the following graphs represents the kinetics of protein precipitation by addition of ammonium sulphate? On the $Y$-axis, [Protein] represents the concentrati...

The concentration (in micromolar) of NADH in a solution with $A_{340}=0.50$ is _________Given data: Path length $=1$ cm; Molar extinction coefficient of NADH $\varepsilon...

The circular dichroism spectra of three proteins $P$, $Q$, and $R$ are given below:Choose the correct combination.$P: \alpha- \text{helix}, Q: \beta- \text{sheet}, \text{...

The specific activity of an enzyme in a crude extract of $\textit{E.coli}$ is $9.5$ units/mg of protein. The specific activity increased to $68$ units/mg of protein upon ...