Recent questions tagged biotechnology

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In a fed-batch culture, $200 \text{ g . L}^{-1}$ glucose solution is added at a flow rate of $50 \text{ L . h}^{-1}$ . The initial culture volume (at quasi steady state) ...
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Which of the following is employed for the repeated use of enzymes in bioprocesses?polymerizationimmobilizationligationisomerization
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The growth medium for mammalian cells contains serum. One of the major functions of serum is to stimulate cell growth and attachment. However, it must be filter sterilize...
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Consider a continuous culture provided with a sterile feed containing $10$ mM glucose. The steady state cell density and substrate concentration at three different diluti...
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Topological Winding number of a $\text{2.0 kb}$ covalently closed circular DNA was found to be $191$ with a writhing number of $-4$. Hence, its $\text{LINKING NUMBER}$ an...
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A fed batch culture was operated with intermittent addition of glucose solution at a flow rate of $\text{200 ml h}^{-1}$. The values of $K_s,\: \mu_m$ and $D$ are $\text{...
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The maximum cell concentration $(\text{g l}^{-1})$ expected in a bioreactor with initial cell concentration of $\text{1.75 g l}^{-1}$ and an initial glucose concentration...
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A callus of $\text{5 g}$ dry weight was inoculated on semi-solid medium for growth. The dry weight of the callus was found to increase by $1.5$ fold after $10$ days of in...
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Match the commercial microbial sources in $\text{Group I}$ with the products in $\text{Group II}$.$\begin{array}{|l|l|l|l|} \hline & \text{Group I} & & \text{Group II} \...
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A chemostat is operated at a dilution rate of $0.6\; h^{-1}$. At steady state, the biomass concentration in the exit stream was found to be $30\; gl^{-1}$The biomass prod...
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An enzymatic reaction is described by the following rate expression.$$v = \frac{v_mS}{K_m+S+S^2/K_s},$$Which one of the following curves represents this expression?
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Match the entries in $\text{Group I}$ with the methods of sterilization in $\text{Group II}$$\begin{array}{|l|l|l|l|} \hline & \textbf{Group I} && \text{Group II} \\ \h...
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Match the downstream processes in $\text{Group I}$ with the products in $\text{Group II}$.$\begin{array}{|C|l|C|l|} \hline& \textbf{Group I} & {} & \textbf{Group II} \\\h...
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Match the entries in $\text{Group I}$ with the process parameters in $\text{Group II}$.$\begin{array}{|C|l|C|l|} \hline &\textbf{Group I} & {} & \text{Group II} \\\hline ...
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Determine the correctness or otherwise of the following $Assertion$ (a) and $Reason$ (r).$Assertion:$ Cell mass yield of a methylotrophic yeast is more on methanol compar...
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Which one of the following is NOT a protoplast fusion inducing agent?Inactivated Sendai virus$Ca^{2+}$ at alkaline pHPolyethylene glycolColchicine
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Match the industrial products mentioned in Group I with their producer organisms in Group II$\begin{array}{|l|l|l|l|} \hline & \textbf{Group I} && \textbf{Group II} \\ \h...
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A single stem cell undergoes $10$ asymmetric cell divisions. The number of stem cells at the end is _________
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Which one of the following plant secondary metabolites is a natural insecticide?DigitoxinPyrethrinSalicylic acidAvenacin A-$1$
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Which one of the following is an example of a neurotoxin?Cholera toxinStreptolysin-OBotulinuin toxinDiphtheria toxin
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If serum is removed from the growth medium of human embryonic kidney cell line (HEK), then the cells willproliferate fasterproliferate normallyundergo cell cycle arrestun...