Recent questions tagged biotechnology

For monoclonal antibody production, hypoxanthine $\_\_\_\_$.allows DNA synthesis in the absence of de novo dTMP synthesisallows purine synthesis in cells containing HGPRT...

The correct sequence of the four steps involved in anaerobic production of biogas from complex organics is:Hydrolysis → Acidogenesis → Acetogenesis → MethanogenesisMethan...

A $\text{CSTR}$ with a volume of $100 \mathrm{~m}^{3}$ is operated in cell recycle mode. At a volumetric flow rate of $10 \mathrm{~m}^{3}$ day ${ }^{-1}$ and effluent bio...

​​​​​Which one of the following hosts is used in mammalian cell culture for the production of glycosylated recombinant therapeutic proteins?Pichia pastorisSf$9$ cellsEsch...

​​​​A recombinant protein is secreted extracellularly in soluble form by an $E. coli$ culture. Which of the following downstream processes is/are involved in the purifica...

Match the industrial enzyme (Column I) with its application (Column II).\begin{array}{|c|c|c|}\hline \text{Column I} & \text{Column II} \\\hline \text{P. Lipase} & 1. \...

Which of the following is/are considered as biotic elicitor(s) in plant cell culture?CellulaseChitinChitosanMercuric chloride

In hybridoma technology, which one of the following enzymes is absent in the myeloma cells that are used for monoclonal antibody production?Hypoxanthine-guanine phosphori...

Match the types of RNA in Group I with their corresponding function in Group II.Group IGroup IIP. mRNA1. Serves as adaptors between mRNA and amino acids during protein sy...

Which of the following statement(s) is(are) TRUE about induced pluripotent stem cells?They can self-renewThey require specific signals to maintain their stemnessThey cann...

Which of the following is(are) plant protoplast fusogenic agent(s)?Sodium nitratePolyvinyl alcoholPolyethylene glycolBromoxynil

Match the media component (Column I) with its role (Column II).$$\begin{array} {ll} \textbf{Column I} & \textbf{Column I} \\ \text{P. Sucrose} & \text{1. Anti-foam agen...

Match the microbial product (Column I) with its respective application (Column II).$$\begin{array} {lll} \textbf{Column I} & & \textbf{Column II} \\ \text{P. Methane} & &...

Which of the following statements are $\text{CORRECT}$ for an enzyme entrapped in a spherical particle?Effectiveness factor is ratio of the reaction rate with diffusion-l...

The amount of biomass in a reactor at the end of the batch process is $50 \; \text{g}.$ Fed-batch operation is initiated by feeding the substrate solution at a constant r...

Match separation methods in $\text{Group I}$ with associated properties in $\text{Group II}$.$$\begin{array}{|l|l|l|l|} \hline & \textbf{Group I} && \textbf{Group II} \\ ...

A batch cultivation of $\textit{E. coli}$ follows zeroth order Monod’s growth kinetics. The cell growth is terminated when the residual dissolved oxygen concentration att...

Which of the following statements is $ALWAYS\; CORRECT$ about an ideal chemostat?Substrate concentration inside the chemostat is equal to that in the exit streamOptimal d...

Some of the cytokinins used in plant tissue culture media are given below:$BAP$ZeatinKinetin$2iP$Which of these are synthetic analogs?$P$ and $Q$ only$Q$ and $S$ only$R$ ...

Which of the following statements about gene therapy are CORRECT?Affected individuals, but not their progeny, can be cured through germline gene therapyAffected individua...

Determine the correctness or otherwise of the following Assertion [$a$] and the Reason [$r$].Assertion [$a$]: A genetically engineered rice that produces beta-carotene in...

The Bt toxin gene from $Bacillus\; thuringiensis$ used to generate genetically modified crops is$cry$$cro$$cdc$$cre$

What will be the binding status of regulatory proteins in lac operon when concentrations of both lactose and glucose are very low in the culture medium?Only the repressor...

Which one of the following is NOT a therapeutic agent based on nucleic acid for the treatment of genetic disorders?Antisense oligonucleotideRibozymeAptamerAvidin

The power required for agitation of non-aerated medium in fermentation is ___________kW.Operating conditions are as follows:Fermentor diameter $= 3$ mNumber of impellers ...

A $1.2$ kb DNA fragment was cloned into $\textit{Bam}$HI and $\textit{Eco}$RI sites located on a $2.8$ kb cloning vector. The $\textit{Bam}$HI and $\textit{Eco}$RI sites ...

An enzyme converts substrate $A$ to product $B$. At a given liquid feed stream of o rate $25$ L.min$^{-1}$ and feed substrate concentration of $2$ mol.L$^{-1}$, the volum...

A recombinant protein is to be expressed under the control of the lac promoter and operator in a strain $\textit{E.coli}$ having the genotype lacI$^+$ crp$^+$ . Even in t...

Which one of the following is a second generation genetically engineered crop?Bt brinjalRoundup soyabeanGolden riceBt rice

Production of monoclonal antibodies by hybridoma technology requiressplenocytesosteocyteshepatocytesthymocytes

Choose the appropriate pair of primers to amplify the following DNA fragment by the polymerase chain reaction (PCR).$$\textrm{5’ – GACCTGTGG- – – – – – – – – – – – – – – ...

Oxygen transfer was measured in a stirred tank bioreactor using dynamic method. The dissolved oxygen tension was found to be $80 \%$ air saturation under steady state con...

Match the microorganism in $\text{Group I}$ with their fermentation products in $\text{Group II}$.$\begin{array}{|l|l|l|l|} \hline & \textbf{Group I} && \textbf{Group II}...

Match the antibiotics in $\text{Group I}$ with their modes of action in $\text{Group II}$.$\begin{array}{|l|l|l|l|} \hline & \text{Group I} && \text{Group II} \\ \hline ...

Biomass is being produced in a continuous stirred tank bioreactor of $750$ L capacity. The sterile feed containing $8 \: g \cdot L^{-1}$ glucose as substrate was fed at a...