Questions without an upvoted answer in Analytical Techniques

Cancer cells display a high level of heterogeneity in the expression of TP$53$ protein. Which of the following techniques can be directly used to determine the intratumor...

Which one of the following proteins would elute third in gel filtration chromatography from a mixture of four proteins given below?Insulin $(6 \text{ kDa})$Myoglobin $(17...

You have purified an enzyme using a series of chromatographic methods. It was observed that a $10 ~\mu \mathrm{g} \mathrm{~mL}^{-1}$ of this purified enzyme converted $10...

The electron equivalent per liter (e-eq $\mathrm{L}^{-1}$ ) of acetate ion solution of concentration $10 \mathrm{~g} \mathrm{~L}^{-1}$ is $\_\_\_\_$ $\mathrm{e}^{-} \math...

​​​​​​Which of the following separation processes is/are based on molecular size?Size-exclusion chromatographyIon exchange chromatographyMembrane ultrafiltrationUltracent...

Within the Michaelis-Menten framework, the ratio of $v_{0} / V_{\max }$ when $[\mathrm{S}]=20 \times K_{\mathrm{m}}$ is $\_\_\_\_\_$(Round off to two decimal places)

The percentage of light that would pass through a sample with an absorbance of $2$ would be $\_\_\_\_\_$ $\%$ .(Round off to the nearest integer)

An NMR spectrometer operating at proton resonance frequency $(v)$ of $1 \: \mathrm{GHz}$ will have a magnetic field strength of $\_\_\_\_$ Tesla (T).The gyromagnetic rati...

For the coupled reactions given below\[\begin{array}{ll}\text { Glucose 6-phosphate }+\mathrm{H}_{2} \mathrm{O} \rightarrow \text { Glucose }+\mathrm{Pi} & \text { (React...

In adsorption chromatography, the adsorption of uncharged solute molecules onto a silica-based stationary phase is by $\_\_\_\_\_\_\_$.covalent bondselectrostatic interac...

Match the item in Column I with the corresponding technique in Column II.\begin{array}{|c|c|c|}\hline & \text{Column I} & \text{Column II} \\\hline & \text{P. Blue laser}...

Which of the following statements is/are correct about an uncompetitive inhibitor of an enzyme?It binds to the substrate binding site of the enzyme onlyIt binds to the en...

In ELISA, which of the following enzymes are conjugated to antibodies for detection of the analyte?P. Alkaline phosphataseQ. TrypsinaseR. Horseradish peroxidaseS. Amylase...

The distance between the two points of intersection of $x^{2}+y=7$ and $x+y=7$ (rounded off to two decimal places) is ___________.

Temperature of a reaction with an activation energy value of $15 \mathrm{kcal} \mathrm{mol}^{-1}$ is increased from $300 \mathrm{~K}$ to $310 \mathrm{~K}$. If the value o...

E. coli cultivated at $298 \mathrm{~K}$ uptakes an uncharged compound (A) by passive diffusion. The intracellular and extracellular concentrations of $\mathrm{A}$ are $0...

If $1000 \mathrm{bp}$ of a double-helical DNA weighs $1 \times 10^{-18} \mathrm{gm}$ and distance between two $\mathrm{bp}$ is $0.34 \mathrm{~nm}$, the total amount of DN...

A protein has three identical sites arranged at the vertices of an equilateral triangle. If one site is filled with a dye (donor), the measured quantum yield $\left(\phi_...

A dNTP master-mix is prepared by combining $40 \mu L$ of each $20 \mathrm{mM}$ dNTP stock (dATP, dCTP, dGTP and dTTP). $4 \mu L$ of this dNTP master-mix is added to a PCR...

Match the stationary phase (Column I) with its corresponding chromatography technique (Column II).$$\begin{array} {lll} \textbf{Column I} & & \textbf{Column II} \\ \text{...

Which of the following spectroscopic technique(s) can be used to identify all the functional groups of an antibiotic contaminant in food?InfraredCircular dichroismNuclear...

Which of the following nucleus/nuclei is/are $\text{NMR}$ active?$^1H$$^{13}C$$^{16}O$$^{32}S$

A $\text{DNA}$ solution of $50 \: \mu g \: mL^{-1}$ concentration gives an absorbance of $1.0$ at $260 \textit{ nm}$. An aliquot of $20 \: \mu L$ from a $50 \: \mu L$ pur...

Protein concentration of a crude enzyme preparation was $10\:mg\:mL^{-1}$. $10\:\mu L$ of this sample gave an activity of $5\:\mu mol\:min^{-1}$ under standard assay cond...

$Group\; I$ lists spectroscopic methods and $Group\; II$ lists biomolecular applications of these methods. Match the methods in $Group\; I$ with the applications in $Grou...

The hexapeptide $P$ has an isoelectric point $(pI)$ of $6.9$. Hexapeptide $Q$ is a variant of $P$ that contains valine instead of glutamate at position $3$. The two pepti...

A UV-visible spectrophotometer has a minimum detectable absorbance of $0.02$. The minimum concentration of a protein sample that can be measured reliably in this instrume...

The difference in concentrations of an uncharged solute between two compartments is $1.6$-fold. The energy required for active transport of the solute across the membrane...

The number of molecules of a nucleotide of molecular weight $300\:g$/mol present in $10$ picomoles is ____________ $\times10^{12}$ (round off to $2$ decimal places).

The concentrations of $ATP$, $ADP$ and inorganic phosphate in a cell are $2.59, \:0.73$ and $2.72 \:mM$, respectively. Under these conditions, free energy change for the ...

Two monomeric His-tagged proteins of identical molecular weight are present in a solution. pIs of these two proteins are $5.6$ and $6.8$. Which one of the following techn...

Solvents $A$ and $B$ are completely immiscible. Solute $S$ is soluble in both these solvents. $100\: g$ of $S$ was added to a container which has $2\: kg$ each of $A$ and...

Which one of the following techniques relies on the spin angular momentum of a photon?CD spectroscopyFluorescence spectroscopyIR spectroscopyRaman spectroscopy

The equilibrium potential of a biological membrane for $Na^+$ is $55$ mV at $37^\circ$ C. Concentration of $Na^+$ inside the cell is $20$ mM. Assuming the membrane is per...

In an assay of the type II dehydroquinase of molecular mass $18$ kDa, it is found that the $V_{\text{max}}$ of the enzyme is $0.0134 \: \mu \text{ mol} \cdot \text{min}^{...

The molar extinction coeffcients of Trp and Tyr at $280$ nm are $5690$ and $1280 \: M^{-1} \cdot cm^{-1}$, respectively. The polypeptide chain of yeast alcohol dehydrogen...

The activity of lactate dehydrogenase can be measured by monitoring the following reaction: $$\text{Pyruvate} + \text{NADH} \rightarrow \text{Lactate} + \text{NAD}^+$$ Th...

A protein is to be purified using ion-exchange column chromatography. The relationship between HETP (Height Equivalent to Theoretical Plate) and the linear liquid velocit...