Recent questions tagged molecular-biology-and-genetics

Which family of viruses has single stranded DNA?HerpesviridaePoxviridaeRetroviridaeParvoviridae

What will be the binding status of regulatory proteins in lac operon when concentrations of both lactose and glucose are very low in the culture medium?Only the repressor...

In a typical mitotic cell division cycle in eukaryotes, $M$ phase occurs immediately after the$G_0$ phase$S$ phase$G_1$ phase$G_2$ phase

Which one of the following is NOT an algorithm for building phylogenetic trees?Maximum parsimonyNeighbor joiningMaximum likelihoodBootstrap

Cesium chloride density gradient centrifugation is commonly used for the separation of DNA molecules. The buoyant density, $\rho$ of a double stranded $Cs^+DNA$ is given ...

Disaccharide molecules that contain $\beta( 1 –\blacktriangleright 4)$ glycosidic linkage aresucrose and maltosesucrose and isomaltosemaltose and isomaltoselactose and ce...

Junctional diversity of antibody molecules results fromthe addition of switch region nucleotidesthe addition of N and P nucleotidesthe joining of V, D and J segmentsmutat...

Based on their function, find the ODD one out.miRNAsiRNAshRNAsnRNA

Which set of the following events occurs during the elongation step of translation?Attachment of mRNA with the smaller subunit of ribosomeLoading of correct aminoacyl-tRN...

A DNA sequence, $\textrm{5’ -ATGGACGTGCTTCCCAAAGCATCGGGC- 3’}$, is mutated to obtain$\textrm{5'-ATGGACGTGCTTC}\textbf{a}\textrm{CAAAGCATCGGGC- 3'}$$\textrm{5'-ATGGACGTGCT...

Select the CORRECT combination of genetic components that are essential for the transfer of T-DNA segment from Agrobacterium tumefaciens to plant cells.Border repeat sequ...

A variety of genetic elements are used in the transgenic plant research. Match the genetic elements (Column-I) with their corresponding source (Column-ll).$\begin{array}{...

Match the type of chromosomal inheritance (Column-I) with the corresponding genetic disease or trait (Column-II).$\begin{array}{|l|l|l|l|} \hline & \textbf{Column-I} & & ...

Which of the following statement(s) is/are CORRECT for antigen activated effector T cells?$CD4^+$ cells make contact with macrophages and stimulate their microbicidal act...

Which one of the following statements regarding G proteins is INCORRECT?GDP is bound to G protein in the resting stageGTP bound $\alpha$ subunit cannot reassemble with $\...

Analysis of a hexapeptide using enzymatic cleavage reveals the following result:Amino acid composition of the peptide is: $2R, A, V, S, Y$Trypsin digestion yields two fra...

If the nucleotide composition $(\%)$ of a viral genome is $A = 10$, $U = 20$, $C = 40$, and $G = 30$, which one of the following is this genome?Double stranded RNASingle ...

Secretory proteins synthesized by ER-associated ribosomes traverse throughmitochondriaperoxisomesthe Golgi apparatusthe nucleus

During protein synthesis, tRNAs are NOT involved incharginginitiationelongationtermination

A proto-oncogene is suspected to have undergone duplication in a certain type of cancer. Of the following techniques, which one would verify the gene duplication?Northern...

A polymerase chain reaction (PCR) was set up with the following reagents: DNA template, Taq polymerase, buffer, dNTPs, and Mg$^{2+}$. Which one of the following is missin...

The transcription factor $X$ binds a $10$ base pair DNA stretch. In the DNA of an organism, $X$ was found to bind at $20$ distinct sites. An analysis of these $20$ bindin...

A bacterium has a genome of size $6$ million base pairs. If the average rate of DNA synthesis is $1000$ base pairs/second, the time taken (in minutes) for replication of ...

Match the proteins in $\textbf{Group I}$ with cellular processes in $\textbf{Group II}$$\begin{array}{|l|l|l|l|} \hline & \textbf{Group I} & & \text{Group II} \\ \hline P...

A recombinant protein is to be expressed under the control of the lac promoter and operator in a strain $\textit{E.coli}$ having the genotype lacI$^+$ crp$^+$ . Even in t...

Shown below id a plasmid vector (P) and an insert (Q). The insert was cloned into the BamHI site of the vector. The recombinant plasmid was isolated and digested with Bam...

A pedigree of an inheritable disease is shown below.This inheritable disease is$X$-linked dominant$X$-linked recessive or Y-linkedonly $Y$-linkedonly $X$-linked recessive

The interaction energy $E$ between two spherical particles is plotted as a function of the distance $(r)$ between them. When $r<a$, where $a$ is a constant, the net force...

A DNA strand of length $25$ nm wraps diametrically around the circumference of a spherical histone-octamer once. The radius (nm) of the histone-octamer is _______Given da...

Which one of the following features is NOT required in a prokaryotic expression vector?$\textit{oriC}$Selection markerCMV promoterRibosome binding site

Which one of the following is INCORRECT about a typical apoptotic cell?Phosphatidylserine is presented on the outer cell surfaceCytochrome c is released from mitochondria...

Identify the file format given below:$>$P$1$; JMFDProtein X – Homo sapiensMKALTARQQEVFDLIRDHISRTLRQQGDWLGDEFASTANBRFGCG

Choose the appropriate pair of primers to amplify the following DNA fragment by the polymerase chain reaction (PCR).$$\textrm{5’ – GACCTGTGG- – – – – – – – – – – – – – – ...

How many rooted and unrooted phylogenetic trees, respectively, are possible with four different sequences?$3$ and $15$$15$ and $3$$15$ and $12$$12$ and $3$

An $\textit{in vitro}$ translation system can synthesize peptides m all three reading frames of the RNA template. When $\text{5’ - UCUCUCUC - (UC)_n -UCUCUCUC - 3’}$ was...

Cytoplasmic extract from the wild type strain of a bacterium has the ability to convert a colorless substrate $(S)$ to a colored product $(P)$ via three colorless interme...

A linear double stranded DNA of length $8$ kbp has three restriction sites. Each of these can either be a $\textit{Bam}\text{HI}$ or a $\textit{Hae}III$ site. The DNA was...

The $5$’ ends of the mature forms of the prokaryotic mRNAs and tRNAs area triphosphate group in mRNAs and a monophosphate group in tRNAstriphosphate groups in both mRNAs ...

The statistical frequency of the occurrence of a particular restriction enzyme cleavage site that is $6$ bases long can be estimated to beonce every $24$ basesonce every ...

The plasmid DNA was subjected to restriction digestion using the enzyme $\textit{EcoRI}$ and analysed on an agarose gel. Assuming digestion has worked (the enzyme was act...