Recent questions tagged molecular-biology-and-genetics

Cholera toxin increases $cAMP$ levels bymodifying $G_i$ proteinmodifying $G_s$ proteinbinding to adenylate cyclaseactivating $cAMP$ phosphodiesterase

Consider a population of $10,000$ individuals, of which $2500$ are homozygotes (PP) and $3000$ are heterozygotes (Pp) genotype. The frequency of allele p in the populatio...

For their efficient translation, eubacterial $mRNAs$ possess a Shine-Dalgarno sequence for its recognition by an anti-Shine-Dalgarno sequence $(ASD)$ in the ribosomes. Th...

Match the items in $\text{Group I}$ with $\text{Group II}$$\begin{array}{|l|l|l|l|} \hline & \text{Group I} & & \text{Group II} \\ \hline P. & \text{Receptor tyrosine kin...

Match the immunoglobulin class in $\text{Group I}$ with its properties in $\text{Group II}$$\begin{array}{|l|l|l|l|} \hline & \textbf{Group I} && \textbf{Group II} \\ \hl...

In a genetic cross between the genotypes $WWXX$ and $\text{wwxx}$, the following phenotypic distributions were observed among the $F_2$ progeny: $\text{WX, 562; wx, 158; ...

Which of the following statements with respect to the orientation of the nitrogenous bases to the pentose sugars, and the puckering of the sugar, is correct?Anti, and 2’-...

The amino acid substitution matrices in decreasing order of stringency for comparing protein sequences arePAM$250$, PAM$120$, PAM$100$PAM$100$, PAM$120$, PAN$250$PAM$250$...

A DNA fragment of $5000bp$ needs to be isolated from E.coli (genome size $4\times10^3kb$) genomic library.The number of clones to represent this fragment in genomic libra...

A DNA fragment of 5000bp needs to be isolated from $E.coli$ (genome size $4\times10^3 kb$) genomic library.The minimum number of independent recombinant clones required t...

Determine the correctness or otherwise of the following $\text{Assertion (a) and Reason (r)}$.$\text{Assertion:}$ $\text{UPGMA}$ method produces ultrametric tree.$\text{R...

Match the entries in the $\text{Group I}$ with the entries in $\text{Group II}$.$\begin{array}{|l|l|l|l|} \hline & \text{Group I} && \text{Group II} \\ \hline P. & \text{...

Match the entries in $\text{Group I}$ with the entries in $\text{Group II}$.$\begin{array}{|l|l|l|l|} \hline & \text{Group I} && \text{Group II} \\ \hline P. & \text{RNA...

Hypophosphatemia is manifested by an $X-$linked dominant allele.What proportion of the offsprings from a normal male and an affected heterozygous female will manifest the...

The suitable substitution matrix to align closely related sequences is$\text{PAM 250 or BLOSSUM 80}$$\text{PAM 40 or BLOSSUM 80}$$\text{PAM 120 or BLOSSUM 40}$$\text{PAM ...

The $RNA$ primer synthesized during the replication process in bacteria is removed by$DNA$ gyraseprimase$DNA$ polymerase $I$$DNA$ polymerase $II$

Of the two diploid species, species $I$ has $36$ chromosomes and species $II$ has $28$ chromosomes. How many chromosomes would be found in an allotriploid individual?$42$...

Which one of the following is an $ABC$ transporter?multidrug resistance proteinacetylcholine receptorbacteriorhodopsin$ATP$ synthase

Phylum proteobacteria is subdivided into $\alpha-, \beta-, \gamma-, \delta-$ and $\epsilon-$ proteobacteria based on$G+C$ content$23S\; rRNA$ sequences$tRNA$ sequences$16...

In nature, the horizontal gene transfer across bacteria is mediated bygene cloning followed by transformationconjugation and transformationconjugation onlytransformation ...

The nucleotide analogue used in DNA sequencing by chain termination method is$l',3'-$dideoxy nucleoside triphosphate$2',3'-$dideoxy nucleoside triphosphate$2',4'-$dideoxy...

Human genome sequencing project involved the construction of genomic library inbacterial artificial chromosome$pBR322$bacteriophage$pcDNA3.1$

Endogenous antigens are presented on to the cell surface along with$MHC-II$$MHC-I$$F_c\gamma$receptorcomplement receptor

Which one of the following modifications is common toboth protein and DNA?SUMOylationNitrosylationMethylationUbiquitination

Under alkaline conditions, DNA is more stable than RNA becauseRNA forms secondary structuresRNA is a single stranded moleculeRNA has uracil in place of thymidineRNA is su...

$\text{Statement for Linked Answer Questions 52 and 53:}$An E $coli$ cell of volume $10^{-12}$ $cm^3$ contains 60 molecules of lac-repressor. The repressor has a binding ...

$\text{Statement for Linked Answer Questions 52 and 53:}$An E $coli$ cell of volume $10^{-12}$ $cm^3$ contains 60 molecules of lac-repressor. The repressor has a binding ...

Determine the correctness or otherwise of the following $Assertion$ (a) and $Reason$ (r).$Assertion:$ In direct somatic embryogenesis, embryos are developed without going...

In zinc finger proteins, the amino acid residues that coordinate zinc areCys and HisAsp and GluArg and LysAsp and Arg

Which one of the following DNA sequences carries an invert repeat?ATGAGCCCCGAGTATACTCGGGGCTCATATGAGCCGGCTCTATACTCGGCCGAGATATGAGCCGAGCCTAACTCGGCTCGGATATGAGCCTATGGTATACTCGG...

Synteny refers togene duplication from a common ancestora tree representation of related sequencesthe extent of similarity between two sequenceslocal conservation of gene...

An example of a program for constructing a phylogenetic tree isphylipphrapprodomPHDsec

An example of a derived protein structure database isPfamSCOPGEOProsite

During photorespiration under low $CO_2$ and high $O_2$ levels, $O_2$ reacts with ribulose1,5- bisphosphate to yieldone molecule each of 3-phosphoglycerate and 2-phosphog...

The direction of shell coiling in the snail $\text{Limnaea peregra}$ is a classic example ofchromosomal inheritanceextra-chromosomal inheritancechromosomal translocationh...

Protein-DNA interactions $\text{in vivo}$ can be studied bygel shift assaySouthern hybridizationchromatin immunoprecipitation assayfluorescence $\text{in situ}$ hybridiza...

A truncated polypeptide is synthesized due to a nonsense mutation. Where would you introduce another mutation to obtain a full-length polypeptide?Ribosomal protein geneTr...

A protein is phosphorylated at a serine residue. A phosphomimic mutant of the protein can be generated by substituting that serine withglycinealanineaspartatethreonine

Choose the correct signal transduction pathway.Hormone $\to$ 7 TM receptor $\to$ G protein $\to$ cAMP $\to$ PKAHormone $\to$ G protein $\to$ 7 TM receptor $\to$ cAMP $\t...

Identification of blood groups involvesprecipitationneutralizationopsonizationagglutination